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首页> 外文期刊>Cogent Food & Agriculture >Hydroxycinnamic acid functional ingredients and their biosynthetic genes in tubers of Solanum tuberosum Group Phureja
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Hydroxycinnamic acid functional ingredients and their biosynthetic genes in tubers of Solanum tuberosum Group Phureja

机译：马铃薯Phureja组块茎中的羟基肉桂酸功能成分及其生物合成基因。

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Potato is an ideal candidate for the delivery of functional ingredients due to its high worldwide consumption. The metabolites in cooked tubers of eight diploid potato genotypes from Colombia were explored. Potato tubers were harvested, cooked，lyophilized, and then stored at ?80°C. Metabolites were extracted from flesh samples and analyzed using liquid chromatography and high-resolution mass spectrometry. A total of 294 metabolites were putatively identified, of which 87 metabolites were associated with health-benefiting roles for humans, such as anticancer and anti-inflammatory properties. Two metabolites, chlorogenic acid and N-Feruloyltyramine were detected in high abundance and were mapped on to the potato metabolic pathways to predict the related biosynthetic enzymes: hydroxycinnamoyl-CoA quinate transferase (HQT) and tyramine hydroxycinnamoyl transferase (THT), respectively. The coding genes of these enzymes identified nonsynonymous single-nucleotide polymorphisms (nsSNPs) in AC09, AC64, and Russet Burbank, with the highest enzyme stability found in AC09. This is consistent with the highest presence of hydroxycinnamic acids in the AC09 genotype. The metabolites detected at high fold change, their functional ingredient properties, and their enhancement through breeding to improve health of the indigenous communities’ of Colombia are discussed.
机译：由于马铃薯在全球范围内的大量消费，因此它是输送功能性成分的理想选择。研究了来自哥伦比亚的八种二倍体马铃薯基因型的煮熟块茎中的代谢物。收获马铃薯块茎，煮熟，冻干，然后在80°C下保存。从肉样品中提取代谢物，并使用液相色谱和高分辨率质谱分析。总共鉴定出294种代谢物，其中87种代谢物与人类的健康益处有关，例如抗癌和抗炎特性。大量检测到两种代谢物绿原酸和N-Feruloyltyramine，并将其定位到马铃薯的代谢途径上以预测相关的生物合成酶：羟肉桂酰辅酶A奎宁酸转移酶（HQT）和酪胺羟肉桂酰转移酶（THT）。这些酶的编码基因在AC09，AC64和Russet Burbank中鉴定出非同义的单核苷酸多态性（nsSNPs），在AC09中具有最高的酶稳定性。这与AC09基因型中羟基肉桂酸的最高含量是一致的。讨论了以高倍变化检测到的代谢物，其功能成分的性质以及通过繁殖而增强的代谢物，从而改善了哥伦比亚土著社区的健康。

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