• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cogent Chemistry >Validation of high-performance liquid chromatography (HPLC) method for quantitative analysis of histamine in fish and fishery products
【24h】

Validation of high-performance liquid chromatography (HPLC) method for quantitative analysis of histamine in fish and fishery products

机译：高效液相色谱（HPLC）方法用于鱼和鱼产品中组胺定量分析的验证

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A high-performance liquid chromatography method is described for quantitative determination and validation of histamine in fish and fishery product samples. Histamine is extracted from fish/fishery products by homogenizing with tri-chloro acetic acid, separated with Amberlite CG-50 resin and C18-ODS Hypersil reversed phase column at ambient temperature (25°C). Linear standard curves with high correlation coefficients were obtained. An isocratic elution program was used; the total elution time was 10?min. The method was validated by assessing the following aspects; specificity, repeatability, reproducibility, linearity, recovery, limits of detection, limit of quantification and uncertainty. The validated parameters are in good agreement with method and it is a useful tool for determining histamine in fish and fishery products.
机译：描述了一种高效液相色谱法，用于定量测定和验证鱼和渔产品样品中的组胺。通过在三氯乙酸中匀浆从鱼/渔业产品中提取组胺，然后在环境温度（25°C）下用Amberlite CG-50树脂和C18-ODS Hypersil反相柱进行分离。获得了具有高相关系数的线性标准曲线。使用等度洗脱程序。总洗脱时间为10分钟。通过评估以下方面来验证该方法;特异性，可重复性，可重复性，线性，回收率，检测限，定量限和不确定度。验证的参数与方法非常吻合，是确定鱼类和渔业产品中组胺的有用工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号