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Application of Bio-Based Wrinkled Surfaces as Cell Culture Scaffolds

机译：基于生物的起皱表面作为细胞培养支架的应用

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Microscopic surface architectures that can be easily manufactured have been in demand as mechano-structural cues for tissue engineering. Microscopic surface reliefs synthesized by wrinkling were expected as cell culture scaffolds for cell proliferation, control of cellular alignment and differentiation, and spheroid generation. We previously developed bio-based wrinkled films prepared via lignification-mimetic reactions and drying. Although these films are expected as a candidate for cell culture scaffolds, stability and morphology of the wrinkled surfaces in aqueous buffer solutions were not explored. Here, we investigate the surface morphologies of the wrinkled films in phosphate-buffered saline, and their application to 3T3 cell culture. The wrinkled film prepared with the immersion treatment at 40 ?°C maintained its wrinkled structure in phosphate-buffered saline even after five days, although the wrinkles were broadened by hydration of the skin layer. Interestingly, higher cell numbers were observed in the 3T3 cell culture using the wrinkled film than using flat film with the same surface composition. In addition, the high biocompatibility of the wrinkled film was confirmed by in vivo experiments. These results strongly encourage application of the wrinkled film as a mechano-structural cue. Studies of the advanced applications for the wrinkled films are now in progress.
机译：作为组织工程的机械结构线索，已经需要易于制造的微观表面结构。通过皱纹合成的微观表面浮雕有望用作细胞培养支架，用于细胞增殖，控制细胞排列和分化以及球体生成。我们以前开发了通过木质化模拟反应和干燥制备的生物基起皱膜。尽管这些膜有望作为细胞培养支架的候选物，但尚未探索缓冲水溶液中起皱表面的稳定性和形态。在这里，我们调查在磷酸盐缓冲盐水中的皱纹膜的表面形态及其在3T3细胞培养中的应用。尽管通过皮肤层的水合作用使皱纹变宽，但是在40℃下通过浸渍处理制备的皱纹膜在磷酸盐缓冲盐水中仍保持其皱纹结构。有趣的是，在使用褶皱膜的3T3细胞培养物中观察到的细胞数量要比在具有相同表面组成的平面膜中观察到的细胞数量更高。另外，通过体内实验证实了皱纹膜的高生物相容性。这些结果强烈促进了起皱膜作为机械结构线索的应用。皱纹膜的高级应用的研究正在进行中。

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