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首页> 外文期刊>Comparative and functional genomics >Evolution and Cellular Function of Monothiol Glutaredoxins: Involvement in Iron-Sulphur Cluster Assembly
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Evolution and Cellular Function of Monothiol Glutaredoxins: Involvement in Iron-Sulphur Cluster Assembly

机译：单硫醇谷胱甘肽毒素的进化和细胞功能：参与铁-硫簇组装。

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A number of bacterial species, mostly proteobacteria, possess monothiol glutaredoxinshomologous to theSaccharomyces cerevisiaemitochondrial protein Grx5, which isinvolved in iron–sulphur cluster synthesis. Phylogenetic profiling is used to predictthat bacterial monothiol glutaredoxins also participate in the iron–sulphur cluster(ISC) assembly machinery, because their phylogenetic profiles are similar to theprofiles of the bacterial homologues of yeast ISC proteins. High evolutionary co-occurrenceis observed between the Grx5 homologues and the homologues of theYah1 ferredoxin, the scaffold proteins Isa1 and Isa2, the frataxin protein Yfh1 and theNfu1 protein. This suggests that a specific functional interaction exists between theseISC machinery proteins. Physical interaction analyses using low-definition proteindocking predict the formation of strong and specific complexes between Grx5 andseveral components of the yeast ISC machinery. Two-hybrid analysis has confirmedthein vivointeraction between Grx5 and Isa1. Sequence comparison techniques andcladistics indicate that the other two monothiol glutaredoxins ofS. cerevisiae, Grx3and Grx4, have evolved from the fusion of a thioredoxin gene with a monothiolglutaredoxin gene early in the eukaryotic lineage, leading to differential functionalspecialization. While bacteria do not contain these chimaeric glutaredoxins, in manyeukaryotic species Grx5 and Grx3/4-type monothiol glutaredoxins coexist in the cell.
机译：许多细菌种类（大多数是蛋白质细菌）具有与酿酒酵母线粒体蛋白Grx5同源的单硫醇戊二糖谷氨酸毒素，该蛋白涉及铁-硫簇的合成。系统发育谱分析可用于预测细菌单硫醇戊二醛毒素也参与铁硫簇（ISC）组装机制，因为它们的系统发生谱与酵母ISC蛋白的细菌同源谱相似。在Grx5同源物与Yah1铁氧还蛋白，支架蛋白Isa1和Isa2，frataxin蛋白Yfh1和theNfu1蛋白的同源物之间观察到高度进化共现。这表明这些ISC机制蛋白之间存在特定的功能相互作用。使用低清晰度蛋白质对接进行的物理相互作用分析预测，Grx5与酵母ISC机器的几个组件之间将形成强而特定的复合物。两杂交分析已证实了Grx5与Isa1之间的体内相互作用。序列比较技术和分类学表明S的另外两种单硫醇戊二糖毒素。酿酒酵母Grx3和Grx4，是在真核生物谱系早期从硫氧还蛋白基因与单硫羟戊二糖毒素基因的融合演变而来的，从而导致功能差异化。虽然细菌不包含这些嵌合的戊二醛毒素，但在许多真核生物种中，Grx5型和Grx3 / 4型单硫醇戊二醛毒素共存于细胞中。

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