Shotgun Cloning of Transposon Insertions in the Genome ofCaenorhabditis elegans

Alexander M.van der Linden; Ronald H. A.Plasterk

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Shotgun Cloning of Transposon Insertions in the Genome ofCaenorhabditis elegans

机译：秀丽隐杆线虫基因组中转座子插入的Shot弹枪克隆

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We present a strategy to identify and map large numbers of transposon insertions inthe genome ofCaenorhabditis elegans. Our approach makes use of the mutator strainmut-7, which has germline-transposition activity of the Tc1/mariner family of transposons,a display protocol to detect new transposon insertions, and the availability ofthe genomic sequence ofC. elegans. From a pilot insertional mutagenesis screen, wehave obtained 351 new Tc1 transposons inserted in or near 219 predictedC. elegansgenes. The strategy presented provides an approach to isolate insertions of naturaltransposable elements in manyC. elegansgenes and to create a large-scale collectionofC. elegansmutants.

机译：我们提出一种策略，以识别和定位秀丽隐杆线虫基因组中的大量转座子插入。我们的方法利用了具有变种Tc1 / mariner转座子的种系转座活性的mutator mut-7，检测新转座子插入的展示方案以及C基因组序列的可用性。线虫。从试点插入诱变筛选，我们已经获得了351个新的Tc1转座子，它们插入到219 C或附近。线虫基因。提出的策略提供了一种方法来隔离许多C中自然可转座元件的插入。线虫基因并创建大规模的C集合。线粒体突变体。

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《Comparative and functional genomics》 |2004年第3期|共5页
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Alexander M.van der Linden; Ronald H. A.Plasterk;
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1. Shotgun cloning of transposon insertions in the genome of Caenorhabditis elegans [J] . Alexander M. van der Linden, Ronald H. A. Plasterk Comparative and functional genomics . 2004,第3期

机译：秀丽隐杆线虫基因组中转座子插入的弹枪克隆
2. Transposon Express, a software application to report the identity of insertions obtained by comprehensive transposon mutagenesis of sequenced genomes: analysis of the preference for in vitro Tn5 transposition into GC-rich DNA - art. no. e113 [J] . Herron PR, Hughes G, Chandra G, Nucleic Acids Research . 2004,第14期

机译：Transposon Express，一种软件应用程序，用于报告通过测序基因组的全面转座子诱变获得的插入物的身份：分析将体外Tn5换位为富含GC的DNA的偏好-技术。没有。 e113
3. Transposon Express, a software application to report the identity of insertions obtained by comprehensive transposon mutagenesis of sequenced genomes: analysis of the preference for in vitro Tn5 transposition into GC-rich DNA [J] . Gareth Hughes, Govind Chandra, Paul J. Dyson, Nucleic acids research . 2004,第14期

机译：Transposon Express，一种软件应用程序，用于报告通过序列基因组的全面转座子诱变获得的插入物的身份：分析将体外Tn5转置为富含GC的DNA的偏好
4. Hot Clones: A Shotgun Marriage of Search-Driven Development and Clone Management [C] . Schwarz N. Software Maintenance and Reengineering (CSMR), 2012 16th European Conference on . 2012

机译：热克隆：搜索驱动开发和克隆管理的A弹枪婚姻
5. Identification, cloning, sequencing, and characterization of a proposed lactacin B enhancer protein and partial characterization of the Lactobacillus delbrueckii ssp. lactis ATCC 4797 genome by shotgun cloning and random sequencing. [D] . Ulrich, Ricky Lee. 1999

机译：鉴定，克隆，测序和拟议的lactacin B增强蛋白的表征以及德氏乳杆菌的部分表征。通过散弹枪克隆和随机测序获得乳酸ATCC 4797基因组。
6. Shotgun Cloning of Transposon Insertions in the Genome of Caenorhabditis elegans [O] . Alexander M. van der Linden, Ronald H. A. Plasterk 2004

机译：秀丽隐杆线虫基因组中转座子插入的弹枪克隆
7. Shotgun Cloning of Transposon Insertions in the Genome of Caenorhabditis elegans [O] . Ronald H. A. Plasterk, Alexander M. van der Linden 2006

机译：克隆在秀丽隐杆线虫基因组中转座子插入的霰弹枪

Shotgun Cloning of Transposon Insertions in the Genome ofCaenorhabditis elegans

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