Phenotypic Analysis of C57BL/6J and FVB/NJ Mice Generated Using Evaporatively Dried Spermatozoa

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Phenotypic Analysis of C57BL/6J and FVB/NJ Mice Generated Using Evaporatively Dried Spermatozoa

机译：蒸发干燥精子产生的C57BL / 6J和FVB / NJ小鼠的表型分析

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Combinationofevaporativedryingandfrozenstorageat#8211;80°ChasbeenusedsuccessfullytopreservehybridB6D2F1mousespermatozoa.Todeterminewhetherthismethodcanbeappliedequallywelltoinbredmice,spermatozoaofC57BL/6JandFVB/NJmicewereevaporativelydriedandstoredfor1moat#8211;80°Cbeforebeingusedforintracytoplasmicsperminjection(ICSI)toproduceliveoffspring.Afterweaning,1maleand1femalemousefromeachlitterwererandomlyselectedat8wkofagefornaturalmatingtoproduceliveoffspring.Resultsshowedthatspermatozoafrombothinbredstrainsthathadbeenevaporativelydriedandsubsequentlystoredat#8211;80°Ccouldbeusedsuccessfullytoderivelive,healthy,andreproductivelysoundoffspringbyICSI.Nosignificantdifferenceswerefoundinembryotransferrate(numberofpupsbornumberofembryostransferred),littersize,weaningrate,bodyweight,numberofpathologiclesions,andamountofcontaminationbypathogensofmiceproducedbyICSIusingevaporativelydriedspermatozoacomparedwithmiceproducedbynaturalmatingorbyICSIusingfresh(thatis,nonpreserved)spermatozoa.ProgenyproducedbymatingmicegeneratedfromICSIusingevaporativelydriedspermatozoawerenormal.Therefore,spermatozoafrominbredmousestrainsC57BL/6JandFVB/NJcanbepreservedsuccessfullyafterevaporativedryingandfrozenstorageat#8211;80°C.

机译：Combinationofevaporativedryingandfrozenstorageat＃8211; 80℃ChasbeenusedsuccessfullytopreservehybridB6D2F1mousespermatozoa.Todeterminewhetherthismethodcanbeappliedequallywelltoinbredmice，spermatozoaofC57BL / 6JandFVB / NJmicewereevaporativelydriedandstoredfor1moat＃8211; 80℃Cbeforebeingusedforintracytoplasmicsperminjection（ICSI）toproduceliveoffspring.Afterweaning，1maleand1femalemousefromeachlitterwererandomlyselectedat8wkofagefornaturalmatingtoproduceliveoffspring.Resultsshowedthatspermatozoafrombothinbredstrainsthathadbeenevaporativelydriedandsubsequentlystoredat＃8211; 80℃Ccouldbeusedsuccessfullytoderivelive，健康，andreproductivelysoundoffspringbyICSI.Nosignificantdifferenceswerefoundinembryotransferrate（numberofpupsborn / numberofembryostransferred），littersize ，断奶率，体重，病理病变数，和使用天然（或未保存）精子的自然交配或通过ICS（天然）保存的小鼠相比，ICSI使用蒸发干燥的精子生产的小鼠的病原体污染程度和污染程度。 ICS的配对小鼠使用蒸发干燥的精子进行的繁殖是正常的。因此，在80°C下进行蒸发干燥和冷冻保存后，可以成功保存C57BL / 6J和FVB / NJ自交系小鼠的精子。

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《Comparative Medicine》 |2007年第5期|共页
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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1. Phenotypic analysis of C57BL/6J and FVB/NJ mice generated using evaporatively dried spermatozoa [J] . Li MW, Biggers JD, Toner M, Comparative Medicine . 2007,第5期

机译：用蒸发干燥的精子产生的C57BL / 6J和FVB / NJ小鼠的表型分析
2. Behavioral differences between C57BL/6J x FVB/NJ and C57BL/6J x NZB/B1NJ F1 hybrid mice: relation to control of ethanol intake. [J] . Ozburn AR, Harris RA, Blednov YA Behavior Genetics: An International Journal Devoted to Research in the Inheritance of Behavior in Animals and Man . 2010,第4期

机译：C57BL / 6J x FVB / NJ和C57BL / 6J x NZB / B1NJ F1杂种小鼠之间的行为差异：与乙醇摄入控制的关系。
3. Behavioral Differences Between C57BL/6J × FVB/NJ and C57BL/6J × NZB/B1NJ F1 Hybrid Mice: Relation to Control of Ethanol Intake [J] . A. R. Ozburn, R. A. Harris, Y. A. Blednov Behavior Genetics . 2010,第4期

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4. Comparison of Reference Values in Whole Blood of DMDmdx/J and C57BL/6J Mice Using Neutron Activation Analysis [C] . S. Metairon, C.B. Zamboni, M.F. Suzuki, Brazilian Workshop on Nuclear Physics . 2011

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5. Chemo fog: An analysis of long-term neurobiological effects of chemotherapy and potential neuroprotectants in C57BL/6J mice. [D] . Fessler, Christy Lee. 2014

机译：化学雾：C57BL / 6J小鼠化学疗法和潜在神经保护剂的长期神经生物学作用分析。
6. Behavioral Differences Between C57BL/6J × FVB/NJ and C57BL/6J × NZB/B1NJ F1 Hybrid Mice: Relation to Control of Ethanol Intake [O] . A. R. Ozburn, R. A. Harris, Y. A. Blednov -1

机译：C57BL / 6J×FVB / NJ和C57BL / 6J×NZB / B1NJ F1杂交小鼠之间的行为差异：与乙醇摄入的控制
7. Differential effects of high fat diet and diet-induced obesity on skeletal acquisition in female C57BL/6J vs. FVB/NJ Mice [O] . M.J. Devlin, A. Robbins, M.N. Cosman, 2018

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Phenotypic Analysis of C57BL/6J and FVB/NJ Mice Generated Using Evaporatively Dried Spermatozoa

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