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首页> 外文期刊>Comparative and functional genomics >Comparative Genomics Analysis of Plasmid pPV989-94 from a Clinical Isolate of Pantoea vagans PV989
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Comparative Genomics Analysis of Plasmid pPV989-94 from a Clinical Isolate of Pantoea vagans PV989

机译：泛菌PV989临床分离株质粒pPV989-94的比较基因组学分析

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摘要

Pantoea vagans, a gram-negative bacterium from the genus Pantoea and family Enterobacteriaceae, is present in various natural environments and considered to be plant endophytes. We isolated the Pantoea vagans PV989 strain from the clinic and sequenced its whole genome. Besides a chromosome DNA molecule, it also harboured three large plasmids. A comparative genomics analysis was performed for the smallest plasmid, pPV989-94. It can be divided into four regions, including three conservative regions related to replication (R1), transfer conjugation (R2), and transfer leading (R3), and one variable region (R4). Further analysis showed that pPV989-94 is most similar to plasmids LA637P2 and pEA68 of Erwinia amylovora strains isolated from fruit trees. These three plasmids share three conservative regions (R1, R2, and R3). Interestingly, a fragment (R4) in R4, mediated by phage integrase and phage integrase family site-specific recombinase and encoding 9 genes related to glycometabolism, resistance, and DNA repair, was unique in pPV989-94. Homologues of R4 were found in other plasmids or chromosomes, suggesting that horizontal gene transfer (HGT) occurred among different bacteria of various species or genera. The acquired functional genes may play important roles in the adaptation of bacteria to different hosts or environmental conditions.
机译：Pantoea vagans（一种来自Pantoea属和肠杆菌科的革兰氏阴性细菌）存在于各种自然环境中，被认为是植物内生菌。我们从诊所中分离出了Pantoea vagans PV989菌株，并对其整个基因组进行了测序。除了染色体DNA分子外，它还包含三个大质粒。对最小的质粒pPV989-94进行了比较基因组分析。它可以分为四个区域，包括与复制（R1），转移共轭（R2）和转移前导（R3）有关的三个保守区域和一个可变区（R4）。进一步的分析表明，pPV989-94与从果树中分离得到的解淀粉欧文氏菌菌株的质粒LA637P2和pEA68最相似。这三个质粒共有三个保守区（R1，R2和R3）。有趣的是，在pPV989-94中，由噬菌体整合酶和噬菌体整合酶家族位点特异性重组酶介导并编码与糖代谢，抗性和DNA修复有关的9个基因的R4片段（R4）是独特的。在其他质粒或染色体中发现了R4的同源物，这表明在不同物种或属的不同细菌之间发生了水平基因转移（HGT）。获得的功能基因可能在细菌适应不同宿主或环境条件中起重要作用。

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