Assessment of rpoB and 16S rRNA Genes as Targets for PCR-Based Identification of Pasteurella pneumotropica

Dole Vandana S; Banu Laila A; Fister Richard D; Nicklas Werner; Henderson Kenneths S

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Assessment of rpoB and 16S rRNA Genes as Targets for PCR-Based Identification of Pasteurella pneumotropica

机译：评估rpoB和16S rRNA基因作为基于PCR鉴定嗜热巴斯德氏菌的靶标

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DiagnosisofPasteurellapneumotropicainlaboratoryanimalsreliesonisolationoftheorganism,biochemicalcharacterization,and,morerecently,DNA-baseddiagnosticmethods.16SrRNAandrpoBgenesequenceswereexaminedfordevelopmentofareal-timePCRassay.PartialsequencingofrpoB(456bp)and16SrRNA(1368bp)ofPasteurellapneumotropicaisolatesidentifiedbymicrobiologicandbiochemicalassaysindicatedthateithergenesequencecanbeusedtodistinguishP.pneumotropicafromothermembersofthePasteurellaceaefamily.However,alignmentofrpoBsequencesfromthePasteurellapneumotropicaHeyl(15sequences)andJawetz(16sequences)biotypeswithotherPasteurellaceaesequencesfromGenBankindicatedthatalthoughrpoBDNAsequencingcouldbeusedfordiagnosis,developmentofdiagnosticprimersandprobeswouldbedifficult,becausethesequencevariabilitybetweenHeylandJawetzbiotypesisnotclusteredinanyparticularregionoftherpoBsequence.Incontrast,alignmentof16SrRNAsequencesrevealedaregionwithuniqueandstablenucleotidemotifssufficienttopermitdevelopmentofaspecificfluorogenicreal-timePCRassaytoconfirmP.pneumotropicaisolatedbycultureandtodifferentiateHeylandJawetzbiotypes.

机译：DiagnosisofPasteurellapneumotropicainlaboratoryanimalsreliesonisolationoftheorganism，biochemicalcharacterization，并且，morerecently，DNA-baseddiagnosticmethods.16SrRNAandrpoBgenesequenceswereexaminedfordevelopmentofareal-timePCRassay.PartialsequencingofrpoB（456bp）and16SrRNA（1368bp）ofPasteurellapneumotropicaisolatesidentifiedbymicrobiologicandbiochemicalassaysindicatedthateithergenesequencecanbeusedtodistinguishP.pneumotropicafromothermembersofthePasteurellaceaefamily.However，alignmentofrpoBsequencesfromthePasteurellapneumotropicaHeyl（15sequences）andJawetz（16sequences）biotypeswithotherPasteurellaceaesequencesfromGenBankindicatedthatalthoughrpoBDNAsequencingcouldbeusedfordiagnosis，developmentofdiagnosticprimersandprobeswouldbedifficult，becausethesequencevariabilitybetweenHeylandJawetzbiotypesisnotclusteredinanyparticularregionoftherpoBsequence.Incontrast，alignmentof16SrRNAsequencesrevealedaregionwithuniqueandstablenucleotidemotifssufficienttopermitdevelopmentofaspecificfluorogenicreal-timeP CR测定可通过培养分离出嗜肺性疟原虫并区分Heyland Jawetz生物型。

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《Comparative Medicine》 |2010年第6期|共页
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Dole Vandana S; Banu Laila A; Fister Richard D; Nicklas Werner; Henderson Kenneths S;
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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1. Assessment of rpoB and 16S rRNA Genes as Targets for PCR-Based Identification of Pasteurella pneumotropica [J] . Dole Vandana S., Banu Laila A., Fister Richard D., Comparative Medicine . 2010,第6期

机译：评估rpoB和16S rRNA基因作为基于PCR鉴定嗜热巴斯德氏菌的靶标
2. RNA polymerase beta subunit (rpoB) gene and the 16S-23S rRNA intergenic transcribed spacer region (ITS) as complementary molecular markers in addition to the 16S rRNA gene for phylogenetic analysis and identification of the species of the family Mycoplasmataceae [J] . Volokhov D.V., Simonyan V., Davidson M.K., Molecular phylogenetics and evolution . 2012,第1期

机译：除了16S rRNA基因外，RNA聚合酶β亚基（rpoB）基因和16S-23S rRNA基因间转录间隔区（ITS）作为互补分子标记，可用于系统分析和鉴定支原体科的物种
3. Identification of nontuberculous mycobacteria isolated from Hanwoo (Bos taurus coreanae) in South Korea by sequencing analysis targeting hsp65, rpoB and 16S rRNA genes [J] . Kim Bo-Ram, Kim Jae Myung, Kim Byoung-Jun, Veterinary Microbiology . 2014,第3a4期

机译：通过靶向hsp65，rpoB和16S rRNA基因的测序分析鉴定从韩国Hanwoo（Bos taurus coreanae）分离的非结核分枝杆菌
4. Assessment of Anaerobic Benzene Degradation Potential Using 16S rRNA Gene-Targeted Real-Time PCR (Abstract) [C] . Marcio L.B. Da Silva, Pedro J.J. Alvarez International Conference on Remediation of Chlorinated adn Recalcitrant Compounds . 2006

机译：使用16S rRNA基因靶向实时PCR评估厌氧苯降解潜力（摘要）
5. Bacterial Species Diversity in Dental Plaque Using rpoB [D] . Le, Rosalin. 2014

机译：利用rpoB
6. Assessment of rpoB and 16S rRNA Genes as Targets for PCR-Based Identification of Pasteurella pneumotropica [O] . Vandana S Dole, Laila A Banu, Richard D Fister, 2010

机译：评估rpoB和16S rRNA基因作为基于PCR鉴定嗜热巴斯德氏菌的靶标
7. Genetic Diversity of Pasteurella dagmatis as Assessed by Analysis of the 16S rRNA and rpoB Gene Sequences [O] . Jarosław Król, Jacek Bania, Magdalena Florek, 2011

机译：通过分析16S rRNA和rpoB基因序列来评估达氏巴斯德氏菌的遗传多样性

Assessment of rpoB and 16S rRNA Genes as Targets for PCR-Based Identification of Pasteurella pneumotropica

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