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首页> 外文期刊>Comparative and functional genomics >PASSIOMA: Exploring Expressed Sequence Tags during Flower Development inPassifloraspp.
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PASSIOMA: Exploring Expressed Sequence Tags during Flower Development inPassifloraspp.

机译：PASSIOMA：在Passifloraspp的花发育过程中探索表达的序列标签。

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摘要

The genusPassifloraprovides a remarkable example of floral complexity and diversity. The extreme variation ofPassifloraflower morphologies allowed a wide range of interactions with pollinators to evolve. We used the analysis of expressed sequence tags (ESTs) as an approach for the characterization of genes expressed duringPassiflorareproductive development. Analyzing thePassiflorafloral EST database (named PASSIOMA), we found sequences showing significant sequence similarity to genes known to be involved in reproductive development such as MADS-box genes. Some of these sequences were studied using RT-PCR andin situhybridization confirming their expression duringPassifloraflower development. The detection of these novel sequences can contribute to the development of EST-based markers for important agronomic traits as well as to the establishment of genomic tools to study the naturally occurring floral diversity amongPassifloraspecies.
机译：西番莲属（Passiflora）提供了花卉复杂性和多样性的杰出例子。西番莲花形态的极端变化使得与传粉媒介的广泛相互作用得以发展。我们使用表达的序列标签（ESTs）的分析作为表征西番莲生殖生产过程中表达的基因的一种方法。分析花椰菜EST数据库（命名为PASSIOMA），我们发现序列与已知参与生殖发育的基因（如MADS-box基因）显示出显着的序列相似性。使用RT-PCR研究了其中的一些序列，并在原位杂交中证实了它们在西番莲花发育过程中的表达。这些新序列的检测可以促进重要农艺性状的基于EST的标记的开发，并有助于建立基因组工具来研究西番莲种间自然发生的花的多样性。

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