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首页> 外文期刊>Comparative and functional genomics >Statistical Analysis of Microarray Data with Replicated Spots: A Case Study withSynechococcusWH8102
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Statistical Analysis of Microarray Data with Replicated Spots: A Case Study withSynechococcusWH8102

机译：具有重复斑点的微阵列数据的统计分析：以SynechococcusWH8102为例

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Until recently microarray experiments often involved relatively few arrays with only a single representation of each gene on each array. A complete genome microarray with multiple spots per gene (spread out spatially across the array) was developed in order to compare the gene expression of a marine cyanobacterium and a knockout mutant strain in a defined artificial seawater medium. Statistical methods were developed for analysis in the special situation of this case study where there is gene replication within an array and where relatively few arrays are used, which can be the case with current array technology. Due in part to the replication within an array, it was possible to detect very small changes in the levels of expression between the wild type and mutant strains. One interesting biological outcome of this experiment is the indication of the extent to which the phosphorus regulatory system of this cyanobacterium affects the expression of multiple genes beyond those strictly involved in phosphorus acquisition.
机译：直到最近，微阵列实验经常涉及相对较少的阵列，每个阵列上每个基因仅具有单个表示。开发了一个完整的基因组微阵列，每个基因具有多个斑点（在整个阵列中空间分布），以便比较海洋蓝细菌和敲除突变株在定义的人工海水培养基中的基因表达。在本案例研究的特殊情况下，开发了用于分析的统计方法，在这种特殊情况下，阵列中存在基因复制，并且使用的阵列相对较少，当前的阵列技术就是这种情况。部分由于阵列内的复制，有可能检测到野生型和突变菌株之间表达水平的很小变化。该实验的一个有趣的生物学结果是表明该蓝细菌的磷调节系统在多大程度上影响了除严格参与磷吸收的基因之外的多个基因的表达。

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