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首页> 外文期刊>Biology Open >Vestigial-like 3 is a novel Ets1 interacting partner and regulates trigeminal nerve formation and cranial neural crest migration
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Vestigial-like 3 is a novel Ets1 interacting partner and regulates trigeminal nerve formation and cranial neural crest migration

机译：Vestigial-like 3是新型的Ets1相互作用伴侣，可调节三叉神经的形成和颅神经c的迁移

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The transcription factor ZmbHLH16, the maize ortholog of OsTIP2 (OsbHLH142), was isolated in the present study. Tissue expression analysis showed that ZmbHLH16 is preferentially expressed in male reproductive organs. Subcellular location analysis of ZmbHLH16 via rice protoplast indicated that it is located in the nucleus. Through nucleotide variation analysis, 36 polymorphic sites in ZmbHLH16, including 23 single nucleotide polymorphisms and 13 InDels, were detected among 78 maize inbred lines. Neutrality tests and linkage disequilibrium analysis showed that ZmbHLH16 experienced no significant evolutionary pressure. Yeast one-hybrid experiment showed that the first 80 residues in the N-terminus of ZmbHLH16 had transactivation activity, whereas the full length did not. Genome-wide coexpression analysis showed that 395 genes were coexpressed with ZmbHLH16. Among these genes, the transcription factor ZmbHLH51 had similar expression pattern and identical subcellular localization to those of ZmbHLH16. Subsequently, the interaction between ZmbHLH51 and ZmbHLH16 was verified by yeast two-hybrid experiment. Through yeast two-hybrid analysis of series truncated ZmbHLH16 fragments, we found not only the typical bHLH domain [175-221 amino acids (a.a.)], but also that the 81-160?a.a. and 241-365?a.a. of ZmbHLH16 could interact with ZmbHLH51. All these results lay the foundation for further understanding the functions of ZmbHLH16.
机译：转录因子ZmbHLH16，OsTIP2的玉米直系同源物（OsbHLH142），在本研究中被分离出来。组织表达分析表明，ZmbHLH16在男性生殖器官中优先表达。通过水稻原生质体对ZmbHLH16的亚细胞定位分析表明它位于细胞核中。通过核苷酸变异分析，在78个玉米自交系中检测到ZmbHLH16的36个多态性位点，包括23个单核苷酸多态性和13个InDel。中性测试和连锁不平衡分析表明ZmbHLH16没有明显的进化压力。酵母一杂交实验表明，ZmbHLH16 N末端的前80个残基具有反式激活活性，而全长则没有。全基因组共表达分析表明，395个基因与ZmbHLH16共表达。在这些基因中，转录因子ZmbHLH51具有与ZmbHLH16相似的表达模式和相同的亚细胞定位。随后，通过酵母双杂交实验验证了ZmbHLH51和ZmbHLH16之间的相互作用。通过对系列截短的ZmbHLH16片段进行酵母双杂交分析，我们不仅发现了典型的bHLH结构域[175-221个氨基酸（a.a.）]，而且发现了81-160Δa.a。和241-365？a.a。 ZmbHLH16可以与ZmbHLH51交互。所有这些结果为进一步了解ZmbHLH16的功能奠定了基础。

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