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首页> 外文期刊>Biology Open >Surface expression marker profile in colon cancer cell lines and sphere-derived cells suggests complexity in CD26+ cancer stem cells subsets
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Surface expression marker profile in colon cancer cell lines and sphere-derived cells suggests complexity in CD26+ cancer stem cells subsets

机译：结肠癌细胞系和球形细胞中的表面表达标志物谱表明CD26 +癌症干细胞亚群的复杂性

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Taking advantage of eight established cell lines from colorectal cancer patients at different stages of the disease and the fact that all of them could form spheres, cell surface biomarkers of cancer stem cells and epithelial-mesenchymal transition were tested. The aim was to investigate cancer stem cells and metastatic stem cells in order to provide functional characterization of circulating tumor cells and promote the development of new anti-metastatic therapies. Our model showed an important heterogeneity in EpCAM, CD133, CD44, LGR5, CD26 and E-cadherin expression. We showed the presence of a subset of E-cadherin+(some cells being E-cadherinhigh) expressing CD26+(or CD26high) together with the well-known CSC markers LGR5 and EpCAMhigh, sometimes in the absence of CD44 or CD133. The already described CD26+/E-cadherinlowornegativeand CD26+/EpCAM?/CD133?subsets were also present. Cell division drastically affected the expression of all markers, in particular E-cadherin, so new-born cells resembled mesenchymal cells in surface staining. CD26 and/or dipeptidyl peptidase 4 inhibitors have already shown anti-metastatic effects in pre-clinical models, and the existence of these CD26+subsets may help further research against cancer metastasis.
机译：利用大肠癌患者在疾病的不同阶段建立的八种细胞系以及它们都可以形成球体这一事实，测试了癌症干细胞的细胞表面生物标志物和上皮-间质转化。目的是研究癌症干细胞和转移性干细胞，以提供循环肿瘤细胞的功能特征并促进新的抗转移疗法的发展。我们的模型在EpCAM，CD133，CD44，LGR5，CD26和E-钙粘蛋白表达中显示出重要的异质性。我们显示存在表达CD26 +（或CD26high）的E-cadherin +（某些细胞为E-cadherinhigh）的子集，以及众所周知的CSC标记LGR5和EpCAMhigh，有时不存在CD44或CD133。还存在已经描述的CD26 + / E-钙粘蛋白低阴性和CD26 + /EpCAMα/CD133β亚基。细胞分裂极大地影响了所有标志物的表达，特别是E-钙粘蛋白，因此新生细胞在表面染色中类似于间充质细胞。 CD26和/或二肽基肽酶4抑制剂已在临床前模型中显示出抗转移作用，这些CD26 +亚集的存在可能有助于进一步研究抗癌转移。

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