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首页> 外文期刊>Journal of Stem Cells and Regenerative Medicine >Expression profiles of cancer stem cell markers in colorectal cancer cell lines
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Expression profiles of cancer stem cell markers in colorectal cancer cell lines

机译：癌症干细胞标志物在大肠癌细胞系中的表达谱

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Background: Cancer stem cells (CSCs) are thought to be responsible for tumor progression and therapy resistance. They have been identified in a variety of human tumors as well as in cancer cell lines. Cell lines might therefore serve as an attractive source for CSC in vitro research. We investigated to which extent colorectal cancer cell lines contain CSC-like cells and if their expression profiles correlate with clinical measures. Methods: Altogether, 12 colorectal cancer cell lines of carcinomas and metastases were analyzed by flow cytometry using a panel of six CSC surface markers (CD326, CD133, CD44, CD166, Msi-1 and Gpr49). Expression frequency of CSC markers was divided into four categories with high (> 70% of cells), moderate (30%), low (1%), and absent (< 1%) expression. Results: All cell lines but one (HT29) showed a stable expression pattern throughout all four replicates. HT29 showed an increased expression for CD133 and CD166 over time and was thus excluded from further analyses. The majority (91%) of cell lines showed high expression for CD326. About half to one third of the cell lines expressed at high frequency CD44 and CD166 (in 45%) and CD133 (in 36%). In contrast, most cell lines expressed Msi1 and Gpr49 at low frequency. Since CD326, Msi1, and Grp49 did not show any major expression differences in between the various cell lines, we checked for potential correlation of CD44, CD133 and CD166 expression differences with clinical parameters. However, we could not observe any significant correlation. Conclusion: Colorectal cancer cell lines do harbour to a substantial amount CSCs. The frequency of such shows a distinct variability among different cell lines particularly for CD44, CD133, and CD166. This might be due to different clinical properties such as tumor progression and metastasizing as reported previously. In our study, case numbers were too small to validate such reports. Interestingly, the frequency of CSC remained considerably stable over multiple passages within the individual cell line, except for HT29. We suggest excluding HT29 from in vitro analyses. In contrast, the remaining 11 cell lines seem to represent stable models of distinct CSC expression profiles and thus can serve for functional, molecular characterization of marker specific expression profiles
机译：背景：癌症干细胞（CSC）被认为是导致肿瘤进展和治疗耐药性的原因。它们已在多种人类肿瘤以及癌细胞系中得到鉴定。因此，细胞系可能成为CSC体外研究的诱人来源。我们调查了大肠癌细胞系在何种程度上含有CSC样细胞，以及它们的表达谱是否与临床指标相关。方法：使用六种CSC表面标记（CD326，CD133，CD44，CD166，Msi-1和Gpr49），通过流式细胞仪分析了总共12种癌和转移的结直肠癌细胞系。 CSC标记的表达频率分为高表达（> 70％的细胞），中表达（30％），低（1％）和不表达（<1％）的四类。结果：除一个细胞（HT29）外，所有细胞系在所有四个重复实验中均表现出稳定的表达模式。 HT29显示CD133和CD166的表达随时间增加，因此被排除在进一步分析之外。大多数细胞系（91％）显示CD326高表达。大约一半到三分之一的细胞系以高频CD44和CD166（占45％）和CD133（占36％）表达。相反，大多数细胞系以低频表达Msi1和Gpr49。由于CD326，Msi1和Grp49在各种细胞系之间未显示任何主要表达差异，因此我们检查了CD44，CD133和CD166表达差异与临床参数之间的潜在相关性。但是，我们无法观察到任何显着的相关性。结论：结直肠癌细胞系确实藏有大量的CSC。这样的频率显示出不同细胞系之间的明显变异性，特别是对于CD44，CD133和CD166。如前所述，这可能是由于不同的临床特性，例如肿瘤进展和转移。在我们的研究中，案例数太少而无法验证此类报告。有趣的是，除HT29外，CSC的频率在单个细胞系中多次传代过程中均保持相当稳定。我们建议从体外分析中排除HT29。相反，剩余的11个细胞系似乎代表了不同CSC表达谱的稳定模型，因此可以用于标记物特异性表达谱的功能性，分子表征

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