PCR Detection of Human Cytomegalovirus DNA in Clinical Specimens Using Novel UL37 Exon 3 and US3 Primers

Marci M. Lesperance; Despina G. Contopoulos-Ioannidis; María del Pilar Gutiérrez; Anamaris M. Colberg-Poley

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PCR Detection of Human Cytomegalovirus DNA in Clinical Specimens Using Novel UL37 Exon 3 and US3 Primers

机译：使用新型UL37外显子3和US3引物的PCR检测临床标本中的人巨细胞病毒DNA

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The sensitivity and specificity of novel UL37 exon 3 (UL37x3) and US3 immediate-early (IE) gene PCR primers to detect human cytomegalovirus (HCMV) DNA in clinical specimens are comparable to those of HCMV DNA polymerase (UL54) primers. The use of these IE primers increases the diagnostic performance of HCMV PCR.

机译：新型UL37外显子3（UL37x3）和US3立即早期（IE）基因PCR引物在临床标本中检测人巨细胞病毒（HCMV）DNA的敏感性和特异性与HCMV DNA聚合酶（UL54）引物相当。这些IE引物的使用增加了HCMV PCR的诊断性能。

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《Clinical and diagnostic laboratory immunology》 |1998年第2期|共3页
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Marci M. Lesperance; Despina G. Contopoulos-Ioannidis; María del Pilar Gutiérrez; Anamaris M. Colberg-Poley;
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中图分类诊断学;
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1. Rapid quantitative PCR assays for the simultaneous detection of herpes simplex virus, varicella zoster virus, cytomegalovirus, Epstein-Barr virus, and human herpesvirus 6 DNA in blood and other clinical specimens. [J] . Engelmann I, Petzold DR, Kosinska A, Journal of Medical Virology . 2008,第3期

机译：快速定量PCR分析法可同时检测血液和其他临床标本中的单纯疱疹病毒，水痘带状疱疹病毒，巨细胞病毒，爱泼斯坦-巴尔病毒和人疱疹病毒6 DNA。
2. Detection of Cytomegalovirus DNA in Human Specimens by LightCycler PCR [J] . Lars Schaade, Peter Kockelkorn, Klaus Ritter, Journal of Clinical Microbiology . 2000,第11期

机译：通过LightCycler PCR检测人标本中的巨细胞病毒DNA
3. Evaluation of a Probe-Based PCR-ELISA System for Simultaneous Semi Quantitative Detection and Genotyping of Human Cytomegalovirus (HCMV) Infection in Clinical Specimens [J] . Majid Talkhabifard, Naeme Javid, Abdolvahab Moradi, The Open Microbiology Journal . 2017,第1期

机译：基于探针的PCR-ELISA系统，用于临床标本中的人巨细胞病毒（HCMV）感染的同时半定量检测和基因分型
4. Microfluidic device for rapid detection of cytomegalovirus (CMV) by sequence-specific hybridization of PCR-amplified CMV-DNA [C] . Briones, M.P., Yamashita, . 2006

机译：通过PCR扩增的CMV-DNA的序列特异性杂交快速检测巨细胞病毒（CMV）的微流体装置
5. Understanding ER-to-mitochondria trafficking of proteins using human cytomegalovirus UL37 proteins [D] . Williamson, Chad D. 2010

机译：使用人类巨细胞病毒UL37蛋白质了解ER向线粒体的蛋白质运输
6. Note: PCR Detection of Human Cytomegalovirus DNA in Clinical Specimens Using Novel UL37 Exon 3 and US3 Primers [O] . Marci M. Lesperance, Despina G. Contopoulos-Ioannidis, María del Pilar Gutiérrez, 1998

机译：注意：使用新型UL37外显子3和US3引物进行PCR检测临床标本中的人类巨细胞病毒DNA
7. PCR Detection of Human Cytomegalovirus DNA in Clinical Specimens Using Novel UL37 Exon 3 and US3 Primers [O] . Marci M. Lesperance, Despina G. Contopoulos-Ioannidis, María del Pilar Gutiérrez, 1998

机译：使用新型UL37外显子3和US3引物在临床标本中的PCR检测人巨细胞病毒DNA

PCR Detection of Human Cytomegalovirus DNA in Clinical Specimens Using Novel UL37 Exon 3 and US3 Primers

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