A Simple Whole-Blood Test for Detecting Antibodies to Human Immunodeficiency Virus

Willfried Schramm; Stephen E. Wade; Gustavo Barriga Angulo; Patricia Castillo Torres; Anthony Burgess-Cassler

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A Simple Whole-Blood Test for Detecting Antibodies to Human Immunodeficiency Virus

机译：用于检测人类免疫缺陷病毒抗体的简单全血测试

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We developed an immunochromatographic whole-blood test (WBT) which detects antibodies to human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2) from fingerstick blood. The sensitivity and specificity of the WBT were 99.41% (1,018 confirmed positive patients) and 99.89% (941 uninfected patients), respectively (enzyme immunoassay [EIA] on serum or plasma as a reference). WBT performance was comparable to those of licensed EIAs and Western blotting, using 18 HIV-2 sera, 23 HIV-1 seroconversion panels, and a low-titer performance panel (in lieu of whole blood).

机译：我们开发了一种免疫色谱全血试验（WBT），可从指尖血液中检测针对1型和2型人类免疫缺陷病毒（HIV-1和HIV-2）的抗体。 WBT的敏感性和特异性分别为99.41％（1,018例确诊阳性患者）和99.89％（941例未感染患者）（以血清或血浆中的酶联免疫测定[EIA]为参考）。使用18个HIV-2血清，23个HIV-1血清转化板和低滴度性能板（代替全血），WBT的性能与获得许可的EIA和Western blotting相当。

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《Clinical and diagnostic laboratory immunology》 |1998年第2期|共3页
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Willfried Schramm; Stephen E. Wade; Gustavo Barriga Angulo; Patricia Castillo Torres; Anthony Burgess-Cassler;
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1. The use of simple, rapid tests to detect antibodies to human immunodeficiency virus types 1 and 2 in pooled serum specimens. [J] . Soroka SD, Granade TC, Phillips S, Journal of clinical virology: The official publication of the Pan American Society for Clinical Virology . 2003,第1期

机译：使用简单，快速的测试来在合并的血清样本中检测针对1型和2型人类免疫缺陷病毒的抗体。
2. A Simple Saliva-Based Test for Detecting Antibodies to Human Immunodeficiency Virus [J] . Willfried Schramm, Gustavo Barriga Angulo, Patricia Castillo Torres, Clinical and diagnostic laboratory immunology . 1999,第4期

机译：一种简单的基于唾液的检测方法，用于检测人类免疫缺陷病毒的抗体
3. Rapid, simple, and reliable doctor's office test for antibodies to human immunodeficiency virus 1 in serum. [J] . A Dafforn, E F Ullman, J D Irvine, Clinical Chemistry: Journal of the American Association for Clinical Chemists . 1990,第7期

机译：快速，简单和可靠的医生办公室测试血清中人类免疫缺陷病毒1抗体的方法。
4. Simultaneous extraction of human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) RNA with the M1000 apparatus does not impair the measurement of the viral load of both viruses when tested by using the LCx PCR system [C] . T. Bourlet, S. Thamm, S. Pillet, European Congress of Virology . 2004

机译：通过使用LCX PCR系统测试，同时提取人免疫缺陷病毒类型1（HIV-1）和丙型肝炎病毒（HCV）RNA，不会损害两种病毒的病毒载量的测量
5. Human immunodeficiency virus type I (HIV-I) envelope evolution and the relationship to neutralizing antibodies. [D] . Blay, Wendy Marie. 2006

机译：I型人类免疫缺陷病毒（HIV-1）的包膜演变以及与中和抗体的关系。
6. Note: A Simple Whole-Blood Test for Detecting Antibodies to Human Immunodeficiency Virus [O] . Willfried Schramm, Stephen E. Wade, Gustavo Barriga Angulo, 1998

机译：注意：用于检测人类免疫缺陷病毒抗体的简单全血测试
7. A Simple Whole-Blood Test for Detecting Antibodies to Human Immunodeficiency Virus [O] . Willfried Schramm, Stephen E. Wade, Gustavo Barriga Angulo, 1998

机译：一种简单的全血测试，用于检测人类免疫缺陷病毒的抗体

A Simple Whole-Blood Test for Detecting Antibodies to Human Immunodeficiency Virus

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