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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Analysis of Specific Immunoglobulin G Subclass Antibodies for Serological Diagnosis of Echinococcosis by a Standard Enzyme-Linked Immunosorbent Assay
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Analysis of Specific Immunoglobulin G Subclass Antibodies for Serological Diagnosis of Echinococcosis by a Standard Enzyme-Linked Immunosorbent Assay

机译：通过标准酶联免疫吸附法分析血清免疫球菌病的特异性免疫球蛋白G亚类抗体

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摘要

The potential roles of specific antibodies of the different immunoglobulin G (IgG) subclasses in the serological diagnosis of cystic echinococcosis (CE) and alveolar echinococcosis (AE) were investigated by an enzyme-linked immunosorbent assay based on hydatid fluid as antigen. Specific antibodies of subclass 1 were found to be of major importance. In sera collected at the time of diagnosis (i.e., before any therapeutic intervention was initiated) they could be demonstrated in 14 of 15 sera from patients with CE and in all 12 sera from patients with AE. The most discriminatory and the most specific antibodies found in this study belonged to IgG subclass 4. Only one false-positive reaction was observed with 253 sera from healthy volunteers, and no cross-reactions occurred in 80 sera from patients with different parasitic infections. Specific IgG4 antibodies could be demonstrated in 61.0 to 66.7% (CE) or 47.6 to 66.7% (AE) of the cases. Antibody levels of IgG subclass 2 were elevated only moderately, and subclass 3 antibodies were detected in a few cases only. In addition, nonspecific reactions in sera of healthy volunteers or patients with other parasitic infections could partially be attributed to antibodies of subclasses 2 and 3.
机译：通过以卵形液为抗原的酶联免疫吸附试验研究了不同免疫球蛋白G（IgG）亚型的特异性抗体在囊性包虫病（CE）和肺泡包虫病（AE）血清学诊断中的潜在作用。发现亚类1的特异性抗体是最重要的。在诊断时（即开始任何治疗干预之前）收集的血清中，可以在15例CE患者的血清中以及14例AE患者的所有血清中证明它们。在这项研究中发现的最具区分性和最特异性的抗体属于IgG亚类4。健康志愿者的253血清仅观察到一个假阳性反应，而来自80种不同寄生虫感染患者的血清中没有发生交叉反应。在61.0％至66.7％（CE）或47.6％至66.7％（AE）的病例中可以证明特异性IgG4抗体。 IgG亚类2的抗体水平仅适度升高，仅在少数情况下检测到亚类3抗体。此外，健康志愿者或患有其他寄生虫感染的患者血清中的非特异性反应可部分归因于亚类2和3的抗体。

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