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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Anti-Trypanosoma cruzi Immunoglobulin G1 Can Be a Useful Tool for Diagnosis and Prognosis of Human Chagas' Disease
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Anti-Trypanosoma cruzi Immunoglobulin G1 Can Be a Useful Tool for Diagnosis and Prognosis of Human Chagas' Disease

机译：抗克氏锥虫免疫球蛋白G1可以作为诊断和诊断人类恰加斯病的有用工具

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Two functionally distinct antibodies, categorized as conventional serology antibodies (CSA) and lytic antibodies (LA) have been described in Chagas' disease, based on their ability to bind to fixed epimastigotes (EPI) or live trypomastigotes (TRYPO), respectively. In this study, the profile of immunoglobulin G (IgG) subclasses of CSA and LA were analyzed by flow cytometry using serum samples from chronic chagasic patients with the indeterminate (IND), cardiac (CARD), and digestive (DIG) clinical forms of the disease. The results were expressed as percentage of positive fluorescent parasites (PPFP) for each sample. CSA showed a higher PPFP than LA for all samples. At serum dilutions between 1:256 and 1:2,048, IgG1 anti-EPI was able to distinguish chagasic from nonchagasic individuals. Different profiles of IgG subclasses were observed for CSA and LA. IgG1 and IgG2 were the main subclasses in CSA, whereas IgG1 and IgG3 were the predominant ones in LA. The reactivity of IgG2 anti-EPI was greater in IND and CARD than in DIG patients. Furthermore, a low level of IgG1 and IgG3 LA was associated with most of the CARD patients. On the other hand, a high level of IgG1 LA was associated with most of the IND patients. In summary, our findings indicate the potential of IgG1 anti-EPI for serological diagnosis of Chagas' disease, providing further evidence for a protective role of LA, and show that IgG1 anti-liveTrypanosoma cruzi TRYPO may be used to predict the risk of cardiac damage in Chagas' disease.
机译：恰加斯氏病中已经描述了两种功能不同的抗体，分别分类为常规血清学抗体（CSA）和溶解性抗体（LA），这是基于它们分别结合固定的表鞭毛纲动物（EPI）或活的类胰鞭毛纲动物（TRYPO）的能力。在这项研究中，CSA和LA的免疫球蛋白G（IgG）亚类的分布通过流式细胞术分析，使用了慢性恰加斯加病患者的血清样本，这些样本的不确定性（IND），心脏（CARD）和消化（DIG）临床形式疾病。结果表示为每个样品的阳性荧光寄生虫（PPFP）的百分比。对于所有样品，CSA的PPFP均高于LA。在1：256和1：2,048之间的血清稀释度下，IgG1抗EPI能够区分无蹄病个体和无蹄病个体。对于CSA和LA，观察到了不同的IgG亚类概况。 IgG1和IgG2是CSA中的主要亚类，而IgG1和IgG3是LA中的主要亚类。在IND和CARD中，IgG2抗EPI的反应性高于DIG患者。此外，低水平的IgG1和IgG3 LA与大多数CARD患者有关。另一方面，大多数IND患者都伴有高水平的IgG1 LA。总之，我们的发现表明IgG1抗EPI在查加斯病血清学诊断中的潜力，为LA的保护作用提供了进一步的证据，并表明IgG1抗活锥虫可能是TRYPO用于预测恰加斯病的心脏损害风险。

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