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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Impaired Bactericidal Activity and Host Resistance to Listeria monocytogenes and Borrelia burgdorferi in Rats Administered an Acute Oral Regimen of Ethanol
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Impaired Bactericidal Activity and Host Resistance to Listeria monocytogenes and Borrelia burgdorferi in Rats Administered an Acute Oral Regimen of Ethanol

机译：急性乙醇口服给药大鼠的杀菌活性和宿主对单核细胞增生性李斯特菌和伯氏疏螺旋体的抵抗力降低

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A rat model was used to examine how ethanol ingestion may interfere with antimicrobial immunity both in vitro and in vivo. Nonimmune Long-Evans rats were given a short-course treatment orally with excessive amounts of ethanol. Their spleens were removed at the time of sacrifice, and separate spleen cell suspensions were prepared and tested in vitro for their ability to kill two bacterial pathogens, Listeria monocytogenes and Borrelia burgdorferi. After the bacteria were mixed separately with various concentrations of spleen cells, it was found that spleen cells from the ethanol-treated rats killed fewer bacteria than matching pair-fed controls, based on counts of the number of cultured CFU (for Listeria) or based on microscopic examination (for Borrelia). For the in vivo studies, ethanol-treated and control rats were infected intraperitoneally with Listeria, and then, 1 to 3 days later, they were assessed for systemic infection based on the numbers of organisms present in their livers and spleens. Numbers of bacterial CFU for both organs were significantly higher in the group fed ethanol for the first 2 days after listerial challenge. These results support the concept that acute exposure to high levels of ethanol can impair host defense mechanisms, especially those expressed at the cellular level, which could lead to increased susceptibility to certain types of infections.
机译：使用大鼠模型检查乙醇摄入在体外和体内如何可能干扰抗微生物免疫。非免疫性Long-Evans大鼠口服过量乙醇进行短期治疗。在处死时去除它们的脾脏，并制备单独的脾细胞悬液并在体外测试其杀死两种细菌病原体的能力，这些细菌病原体是李斯特菌和 Borrelia burgdorferi。将细菌与各种浓度的脾细胞分别混合后，根据培养的CFU数量（针对李斯特菌），发现用乙醇处理的大鼠的脾细胞杀死的细菌比配对喂养的对照少。 ）或基于显微镜检查（用于 Borrelia ）。在体内研究中，乙醇处理的大鼠和对照组的大鼠腹腔内感染了李斯特菌，然后在1至3天后，根据肝脏中存在的生物体的数量对它们进行全身感染评估和脾脏。在利斯特氏菌攻击后的前两天，喂食乙醇的组中两个器官的细菌CFU数量均显着更高。这些结果支持以下观念：急性暴露于高水平的乙醇会损害宿主防御机制，尤其是在细胞水平表达的防御机制，这可能导致对某些类型感染的易感性增加。

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