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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Newly Characterized Species-Specific Immunogenic Chlamydophila pneumoniae Peptide Reactive with Murine Monoclonal and Human Serum Antibodies
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Newly Characterized Species-Specific Immunogenic Chlamydophila pneumoniae Peptide Reactive with Murine Monoclonal and Human Serum Antibodies

机译：新表征的物种特异性免疫原性肺炎衣原体多肽与鼠单克隆和人类血清抗体反应。

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摘要

A monoclonal antibody (MAb) directed against an unknown Chlamydophila pneumoniae epitope has been characterized, and the respective peptide mimotope has been identified. A murine MAb specific for C. pneumoniae was used to select peptides from phage display libraries. The peptides identified from the phage display library clones reacted specifically with the respective target murine MAb and with human sera previously identified as having antibody titers to C. pneumoniae. The selected peptide mimotope sequences tended to be composed of charged residues surrounding a core of hydrophobic residues. The peptide with the best binding could inhibit >95% of binding to the MAb, suggesting that the selected peptide binds the paratope of the respective MAb. The peptide reacted with human sera previously determined by microimmunofluorescence to have anti-C. pneumoniae antibodies. The peptide was competitively competed with the MAb against Renografin-purified, sonicated C. pneumoniae in an enzyme-linked immunosorbent assay and with whole-cell C. pneumoniae in an indirect fluorescence assay format, demonstrating its potential utility in the development of diagnostics. The use of this novel peptide may allow investigators to establish standardized assays free from cross-reactive Chlamydia trachomatis and Chlamydophila psittaci epitopes and immunoreactivity.
机译：已经鉴定了针对未知的肺炎衣原体抗原表位的单克隆抗体（MAb），并且已经鉴定了各自的肽模拟表位。特定于 C 的鼠单克隆抗体。使用肺炎从噬菌体展示文库中选择肽。从噬菌体展示文库克隆鉴定出的肽与各自的靶鼠单克隆抗体和先前鉴定为对 C具有抗体滴度的人血清特异性反应。肺炎。所选的肽模拟表位序列倾向于由围绕疏水性残基核心的带电荷残基组成。具有最佳结合的肽可以抑制> 95％与MAb的结合，这表明所选肽结合了各个MAb的互补位。该肽与先前通过微免疫荧光测定的人血清反应，具有抗-C。肺炎抗体。该肽与MAb竞争着肾上腺素纯化的，超声处理的 C。酶联免疫吸附试验和全细胞 C感染肺炎链球菌。间接荧光测定法显示肺炎，证明其在诊断学开发中的潜在用途。这种新型肽的使用可以使研究者建立无交叉反应的沙眼衣原体和嗜热衣原体抗原表位和免疫反应性的标准化测定方法。

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