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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Antifreeze Solution Improves DNA Recovery by Preserving the Integrity of Pathogen-Infected Blood and Other Tissues
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Antifreeze Solution Improves DNA Recovery by Preserving the Integrity of Pathogen-Infected Blood and Other Tissues

机译：防冻液通过保留病原体感染的血液和其他组织的完整性提高了DNA的回收率

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摘要

Preserving blood samples for shipping and later DNA extraction has been performed by cooling, freezing, drying, freeze-drying, and protease treatment, among other methods. Most methods to preserve field samples for further DNA extraction do not prevent cellular and DNA damage or are useful only in preserving them for short periods. This report introduces a novel method for blood and tissue that allows preservation in freezing temperatures for a prolonged period of time. The solution reported here (20% ethylene glycol-propylene glycol) preserves cells and tissues integrity, as judged by microscopic analysis, and improves DNA yield and quality.
机译：除其他方法外，已经通过冷却，冷冻，干燥，冷冻干燥和蛋白酶处理来保存血液样本以供运输和随后的DNA提取。保留现场样品以进行进一步DNA提取的大多数方法都不能防止细胞和DNA损伤，或者仅在短期内保存有用。该报告介绍了一种用于血液和组织的新方法，该方法可以长时间保存在冷冻温度下。通过显微镜分析判断，此处报道的溶液（20％乙二醇-丙二醇）可保留细胞和组织的完整性，并提高DNA产量和质量。

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