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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Novel Microtechnique for Assessment of Postnatal Maturation of the Phagocytic Function of Neutrophils and Monocytes
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Novel Microtechnique for Assessment of Postnatal Maturation of the Phagocytic Function of Neutrophils and Monocytes

机译：评估中性粒细胞和单核细胞吞噬功能的产后成熟的新型微技术

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We describe a simple test for the evaluation of phagocytosis and provide a chart of reference values to evaluate normal phagocytosis by age. We assessed the postnatal maturation of phagocytic function of neutrophils and monocytes. Phagocytosis was evaluated in newborn children delivered vaginally or by cesarean section, infants, preschool children, schoolchildren, and adult subjects. Two drops of blood were placed on a microscope slide and incubated with Saccharomyces cerevisiae yeasts, and phagocytosis was evaluated by microscopy. Our technique showed results comparable to or better than those obtained by other usual techniques. The neutrophils of newborn children delivered by cesarean section showed a phagocytic capacity 45% higher than those of neonates delivered vaginally, whereas neutrophils from children in the latter group showed the lowest phagocytic capacity of all age groups. Phagocytosis by neutrophils reached the levels seen in adults at about the first year of life, while there were no important variations in phagocytosis by monocytes in the different age groups. The technique described is reliable and fast, uses only a few drops of blood, and allows better preservation of cell function due to the minimal manipulation to which the cells are submitted. The delayed maturation of the phagocytic function by neutrophils may account for the high levels of susceptibility of newborn and infant children to bacterial infections. This practical method of assessment of phagocytosis may allow the diagnosis of primary or secondary phagocytic deficiencies to be made more easily and may allow better monitoring and treatment of those with dysfunctions of these cells.
机译：我们描述了一个简单的测试，以评估吞噬作用，并提供了参考值图表，以按年龄评估正常吞噬作用。我们评估了嗜中性粒细胞和单核细胞吞噬功能的产后成熟度。在阴道或剖宫产新生儿，婴儿，学龄前儿童，学童和成人受试者中评估吞噬作用。将两滴血放在显微镜载玻片上，并与啤酒酵母（Saccharomyces cerevisiae）酵母一起孵育，并通过显微镜对吞噬作用进行评估。我们的技术显示出的结果与其他常用技术相当或更好。剖宫产分娩的新生儿中性粒细胞吞噬能力比经阴道分娩的新生儿中性粒细胞高45％，而后一组的儿童中性粒细胞在所有年龄组中的吞噬能力最低。中性粒细胞的吞噬作用达到了大约第一岁时成人的水平，而在不同年龄组中，单核细胞的吞噬作用没有重要变化。所描述的技术可靠，快速，仅使用几滴血液，并且由于对细胞的操作最少，因此可以更好地保留细胞功能。中性粒细胞吞噬功能的延迟成熟可能是新生儿和婴幼儿对细菌感染的高度敏感性。这种评估吞噬作用的实用方法可以使对原发或继发吞噬缺陷的诊断更加容易，并且可以更好地监测和治疗这些细胞功能障碍的患者。

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