• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and diagnostic laboratory immunology >Competitive-Inhibition Enzyme-Linked Immunosorbent Assay for Detection of Serum Antibodies to Caprine Arthritis-Encephalitis Virus: Diagnostic Tool for Successful Eradication
【24h】

Competitive-Inhibition Enzyme-Linked Immunosorbent Assay for Detection of Serum Antibodies to Caprine Arthritis-Encephalitis Virus: Diagnostic Tool for Successful Eradication

机译：竞争抑制酶联免疫吸附测定法检测对山羊关节炎-脑炎病毒的血清抗体：成功根除的诊断工具

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A competitive-inhibition enzyme-linked immunosorbent assay (cELISA) was evaluated for the detection of serum antibodies to the surface envelope (SU) of caprine arthritis-encephalitis virus (CAEV) in goats. This assay utilized 96-well microtiter plates containing CAEV-63 SU captured by monoclonal antibody (MAb) F7-299 and measured the competitive displacement of horseradish peroxidase-conjugated MAb GPB 74A binding by undiluted goat sera (F. ?zy?rük, W. P. Cheevers, G. A. Hullinger, T. C. McGuire, M. Hutton, and D. P. Knowles, Clin. Diagn. Lab. Immunol. 8:44-51, 2001). Two hundred serum samples from goats in the United States were used to determine the sensitivity and specificity of cELISA based on the immunoprecipitation (IP) of [35S]methionine-labeled viral antigens as a standard of comparison. A positive cELISA was defined as >33.2% inhibition of MAb 74A binding based on 2 standard deviations above the mean percent inhibition of 140 IP-negative serum samples. At this cutoff value, there were 0 of 60 false-negative sera (100% sensitivity) and 5 of 140 false-positive sera (96.4% specificity). Additional studies utilized IP-monitored cELISA to establish a CAEV-free herd of 1,640 dairy goats.
机译：评价了竞争抑制酶联免疫吸附测定（cELISA），以检测山羊山羊关节炎，脑炎病毒（CAEV）的表面包膜（SU）的血清抗体。此测定法利用包含单克隆抗体（MAb）F7-299捕获的CAEV-63 SU的96孔微量滴定板，并测量了未经稀释的山羊血清与辣根过氧化物酶结合的MAb GPB 74A结合的竞争性置换（F.?zy?rük，WP） Cheevers，GA Hullinger，TC McGuire，M.Hutton，和DP Knowles，临床诊断免疫学杂志8：44-51，2001）。以[ 35 S]蛋氨酸标记的病毒抗原的免疫沉淀（IP）为比较标准，以美国山羊的200份血清样品为基础，测定cELISA的敏感性和特异性。基于高于140个IP阴性血清样品的平均抑制百分比的2个标准差，将阳性cELISA定义为对MAb 74A结合的抑制> 33.2％。在此临界值下，假阴性血清60个中有0个（灵敏度为100％），假阳性血清140个中有5个（特异性为96.4％）。其他研究利用IP监测的cELISA建立了无CAEV的1,640只奶山羊群。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号