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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Lyme Borreliosis in Rhesus Macaques: Effects of Corticosteroids on Spirochetal Load and Isotype Switching of Anti-Borrelia burgdorferi Antibody
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Lyme Borreliosis in Rhesus Macaques: Effects of Corticosteroids on Spirochetal Load and Isotype Switching of Anti-Borrelia burgdorferi Antibody

机译：恒河猴猕猴的莱姆病：皮质类固醇对抗伯氏疏螺旋体抗体的螺旋体负荷和同种型转换的影响。

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Experimental Borrelia burgdorferi infection of rhesus monkeys is an excellent model of Lyme disease and closely parallels the infection in humans. Little is known about the interaction of host immunity with the spirochete in patients with chronic infection. We hypothesized that rapid development of anti-B. burgdorferiantibody in immunocompetent nonhuman primates (NHPs) is the major determinant of the reduction of the spirochetal load in Lyme borreliosis. This hypothesis was tested by measurement of the spirochetal load by PCR in association with characterization of the anti-B. burgdorferi humoral immune response in immunocompetent NHPs versus that in corticosteroid-treated NHPs. Although anti-B. burgdorferi immunoglobulin G (IgG) antibody was effectively inhibited in dexamethasone (Dex)-treated NHPs, anti-B. burgdorferi IgM antibody levels continued to rise after the first month and reached levels in excess of IgM levels in immunocompetent NHPs. This vigorous production of anti-B. burgdorferi IgM antibodies was also studied in vitro by measurement of antibody produced by B. burgdorferi-stimulated peripheral blood mononuclear cells. Despite these high IgM antispirochetal antibodies in Dex-treated NHPs, spirochetal loads were much higher in these animals. These data indicate that Dex treatment results in interference with isotype switching in this model and provide evidence that anti-B. burgdorferi IgG antibody is much more effective than IgM antibody in decreasing the spirochetal load in infected animals.
机译：恒河猴的实验性伯氏疏螺旋体感染是莱姆病的极好模型，与人类的感染极为相似。对于慢性感染患者，宿主免疫与螺旋体之间的相互作用了解甚少。我们假设抗 B的迅速发展。具有免疫功能的非人类灵长类动物（NHPs）中的burgdorferi抗体是降低莱姆病（Lyme borreliosis）螺旋体负荷的主要决定因素。通过用PCR测量螺旋体负荷结合抗-em B的表征来检验该假设。具有免疫功能的NHP中的burgdorferi体液免疫反应与皮质类固醇治疗的NHPs相比。尽管抗-B。在地塞米松（Dex）处理的NHPs，抗 B中，burgdorferi免疫球蛋白G（IgG）抗体得到了有效抑制。 burgdorferi IgM抗体水平在第一个月后继续上升，并达到了具有免疫功能的NHP中超过IgM水平的水平。抗 B的这种有力产生。通过测量 B产生的抗体，还对burgdorferi IgM抗体进行了体外研究。 burgdorferi刺激的外周血单个核细胞。尽管在Dex治疗的NHP中具有较高的IgM抗螺旋抗体，但这些动物的螺旋负荷要高得多。这些数据表明，在该模型中，Dex处理导致干扰同种型转换，并提供了抗 B的证据。 burgdorferi IgG抗体在降低感染动物的螺旋体负荷方面比IgM抗体有效得多。

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