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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Detection of Immune Complexes Is Not Independent of Detection of Antibodies in Lyme Disease Patients and Does Not Confirm Active Infection with Borrelia burgdorferi
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Detection of Immune Complexes Is Not Independent of Detection of Antibodies in Lyme Disease Patients and Does Not Confirm Active Infection with Borrelia burgdorferi

机译：免疫复合物的检测并不独立于莱姆病患者的抗体检测，也不能确定伯氏疏螺旋体是否主动感染

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The Borrelia burgdorferi-specific immune complex (IC) test, which uses polyethylene glycol (PEG) precipitation to isolate ICs from serum, has been used as a research test in the laboratory diagnosis of early Lyme disease (LD) and has been proposed as a marker of active infection. We examined whether B. burgdorferi-specific antibodies were present within PEG-precipitated ICs (PEG-ICs) in patients with LD, posttreatment Lyme disease syndrome, and controls, including individuals who received the outer surface protein A (OspA) vaccine. Using a B. burgdorferi whole-cell enzyme-linked immunosorbent assay (ELISA), we obtained positive PEG-IC results not only in patients with a history of LD, but also in individuals vaccinated with OspA vaccine. The frequency of positive PEG-IC ELISAs in OspA vaccinees was significantly higher with ELISA-reactive than with ELISA-negative unprocessed serum samples (P = 0.001), demonstrating dependency between the tests. Similar results were found using samples from rhesus macaques infected with B. burgdorferi, uninfected macaques vaccinated with OspA, and controls. Therefore, testing for the presence of antibodies against B. burgdorferi in PEG-IC preparations is not more likely to reflect active infection than testing in unprocessed serum and should not be used in individuals who received the OspA vaccine.
机译： Borrelia burgdorferi 特异性免疫复合物（IC）测试使用聚乙二醇（PEG）沉淀从血清中分离ICs，已被用作实验室诊断早期莱姆病（LD）的研究测试），并已被提议作为主动感染的标志。我们检查了 B。 LD，治疗后的莱姆病综合症和对照组（包括接受外表面蛋白A（OspA）疫苗的个体）患者的PEG沉淀ICs（PEG-ICs）中存在伯氏疏螺旋体特异性抗体。使用 B。通过burgdorferi全细胞酶联免疫吸附试验（ELISA），我们不仅在具有LD病史的患者中，而且在接种OspA疫苗的个体中均获得了阳性PEG-IC结果。 ELISA反应性的OspA疫苗中阳性PEG-IC ELISA的频率显着高于ELISA阴性的未处理血清样品（ P = 0.001），证明了测试之间的依赖性。使用感染了 B的猕猴的样品发现了相似的结果。 burgdorferi ，接种OspA的未感染猕猴和对照组。因此，测试针对 B的抗体的存在。 PEG-IC制剂中的burgdorferi 与未加工血清中的检测相比，更不可能反映出主动感染，因此不应在接受OspA疫苗的个体中使用。

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