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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Cytokine secretion induced by superantigens in peripheral blood mononuclear cells, lamina propria lymphocytes, and intraepithelial lymphocytes.
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Cytokine secretion induced by superantigens in peripheral blood mononuclear cells, lamina propria lymphocytes, and intraepithelial lymphocytes.

机译：超抗原在外周血单核细胞，固有层淋巴细胞和上皮内淋巴细胞中诱导的细胞因子分泌。

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Superantigens are potent inducers of T-cell proliferation and induce a broad range of cytokines, including tumor necrosis factor (TNF), gamma interferon, and interleukin 2 (IL-2). In the present study, we compared the abilities of different staphylococcal superantigens (staphylococcal enterotoxin B [SEB], staphylococcal enterotoxin E [SEE], and toxic shock syndrome toxin 1 [TSST-1]) to stimulate distinct cytokine profiles in peripheral blood mononuclear cells (PBMC), lamina propria lymphocytes (LPL), and intraepithelial lymphocytes (IEL). One million PBMC, LPL, and IEL were stimulated with various concentrations of superantigen (10 to 0.001 ng/ml) for 24, 48, and 72 h. Maximum cytokine production by PBMC, LPL, and IEL was observed for all three superantigens at 48 h at a concentration of 1 ng/ml. In PBMC, SEE and TSST-1 stimulated more IL-2 and gamma interferon than SEB. SEE and TSST-1 also stimulated more TNF and IL-4 production than SEB. In contrast, SEB stimulated more IL-6 than either SEE or TSST-1. In LPL, there was no SEE-induced IL-2 or IL-4 production, but IL-6, TNF, and gamma interferon were induced. SEB similarly induced no IL-2 or gamma interferon from the LPL, but IL-4, IL-6, and TNF were detected. TSST-1 stimulation of LPL resulted in IL-2 and TNF production but no IL-4, IL-6, or gamma interferon. In IEL, SEE induced no IL-2, IL-4, or gamma interferon but produced IL-6 and TNF, while SEB stimulation resulted in no IL-2 or gamma interferon but did result in detectable IL-4, IL-6, and TNF. Taken together, these data indicate that there are significant differences in the cytokine profiles induced by superantigens in LPL and IEL compared with those in PBMC, and these differences may relate to differences in activation requirements.
机译：超抗原是T细胞增殖的有效诱导剂，可诱导多种细胞因子，包括肿瘤坏死因子（TNF），γ干扰素和白介素2（IL-2）。在本研究中，我们比较了不同葡萄球菌超抗原（葡萄球菌肠毒素B [SEB]，葡萄球菌肠毒素E [SEE]和中毒性休克综合征毒素1 [TSST-1]）刺激外周血单核细胞中不同细胞因子谱的能力。 （PBMC），固有层淋巴细胞（LPL）和上皮内淋巴细胞（IEL）。用各种浓度的超抗原（10至0.001 ng / ml）刺激一百万个PBMC，LPL和IEL，持续24、48和72小时。在48 h时，浓度为1 ng / ml的所有三种超抗原均观察到PBMC，LPL和IEL产生的最大细胞因子。在PBMC中，SEE和TSST-1比SEB刺激更多的IL-2和γ干扰素。 SEE和TSST-1也比SEB刺激更多的TNF和IL-4产生。相反，SEB比SEE或TSST-1刺激更多的IL-6。在LPL中，没有SEE诱导的IL-2或IL-4产生，但是诱导了IL-6，TNF和γ干扰素。 SEB同样没有从LPL诱导出IL-2或γ干扰素，但检测到IL-4，IL-6和TNF。 TSST-1刺激LPL导致产生IL-2和TNF，但没有产生IL-4，IL-6或γ干扰素。在IEL中，SEE不会诱导IL-2，IL-4或γ干扰素，但会产生IL-6和TNF，而SEB刺激不会导致IL-2或γ干扰素，但会导致可检测到的IL-4，IL-6，和TNF。综上所述，这些数据表明，与PBMC中的那些相比，LPL和IEL中的超抗原诱导的细胞因子谱有显着差异，这些差异可能与激活要求的差异有关。

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