...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and diagnostic laboratory immunology >Tuberculin-Purified Protein Derivative-, MPT-64-, and ESAT-6-Stimulated Gamma Interferon Responses in Medical Students before and after Mycobacterium bovis BCG Vaccination and in Patients with Tuberculosis
【24h】

Tuberculin-Purified Protein Derivative-, MPT-64-, and ESAT-6-Stimulated Gamma Interferon Responses in Medical Students before and after Mycobacterium bovis BCG Vaccination and in Patients with Tuberculosis

机译：在牛分枝杆菌卡介苗接种前后，结核病患者中结核菌素纯化的蛋白衍生物，MPT-64和ESAT-6刺激的伽马干扰素反应

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

QuantiFERON-TB (QIFN) (CSL Limited) is a whole-blood assay for the recognition of infection withMycobacterium tuberculosis. QIFN measures gamma interferon (IFN-γ) production when purified protein derivatives (PPDs) of mycobacteria are incubated with venous blood samples. The specificity of QIFN in medical students before and after BCG immunization was assessed, and sensitivity in patients with tuberculosis was assessed. Antigens were PPD derived from M. tuberculosis and twoM. tuberculosis-specific proteins, ESAT-6 and MPT-64. Of 60 medical students, all of whom had 0-mm tuberculin skin tests (TSTs) at study entry, 58 (97%) were initially classified as negative forM. tuberculosis infection by PPD QIFN. Five months after BCG immunization, 7 of 54 students (13%) had a TST result of ≥10 mm and 11 of 54 students (20%) tested positive by PPD QIFN. ESAT-6- and MPT-64-stimulated IFN-γ responses in the medical students were negative prior to and after BCG immunization. For patients with active tuberculosis, 12 of 19 (63%) were positive by PPD QIFN, 11 of 19 (58%) were positive by ESAT-6 QIFN, and 0 of 12 were positive by MPT-64 QIFN. In conclusion, PPD QIFN was negative in 97% of a low-risk population who had not received BCG and who had negative TSTs. The specificities of both the TST and PPD QIFN were reduced following BCG immunization. PPD QIFN and ESAT-6 QIFN were of similar and moderate sensitivity in patients with active tuberculosis, but ESAT-6 QIFN is likely to be more specific because it is not influenced by past BCG exposure.
机译： Quanti FERON- TB （QIFN）（CSL Limited）是一种用于识别结核分枝杆菌感染的全血检测方法。当分枝杆菌的纯化蛋白衍生物（PPD）与静脉血样品一起孵育时，QIFN可以测量γ干扰素（IFN-γ）的产生。评估了卡介苗免疫前后QIFN在医学生中的特异性，并评估了肺结核患者的敏感性。抗原是源自 M的PPD。结核病和两个 M。结核特异性蛋白ESAT-6和MPT-64。在60名医学生中，所有在研究入学时均接受0毫米结核菌素皮肤测试（TST）的学生中，最初将58名（97％）归为em阴性。 PPD QIFN感染结核病。 BCG免疫后五个月，54名学生中的7名（13％）的TST结果≥10 mm，54名学生中的11名（20％）的PPD QIFN检测为阳性。在接受BCG免疫前后，医学生的ESAT-6和MPT-64刺激的IFN-γ反应均为阴性。对于活动性肺结核患者，PPD QIFN阳性的患者中有12名（19％）（63％），ESAT-6 QIFN阳性的19名中有11名（58％），MPT-64 QIFN阳性的12名中有0名。总之，在没有接受BCG且TST阴性的低危人群中，PPD QIFN阴性，占97％。卡介苗免疫后，TST和PPD QIFN的特异性均降低。 PPD QIFN和ESAT-6 QIFN在活动性肺结核患者中具有相似和中等的敏感性，但是ESAT-6 QIFN可能更具特异性，因为它不受过去BCG暴露的影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号