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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Evaluation of immunoassays for detection of antibodies to human herpesvirus 7.
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Evaluation of immunoassays for detection of antibodies to human herpesvirus 7.

机译：评估用于检测人疱疹病毒抗体的免疫分析方法的评估7。

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An enzyme immunoassay (EIA), an immunoblot assay (IB), and an indirect immunofluorescence assay were developed for detection of human herpesvirus 7 (HHV-7) antibodies in human serum. Cross-absorption studies with EIA or IFA using HHV-7 and human herpesvirus 6 (HHV-6) antigens indicated that most human sera contain cross-reactive HHV-6 and HHV-7 antibodies and that the degree of cross-reactivity varies between individual serum specimens. Inhibition of homologous antibody activity by absorption with heterologous virus ranged from 0 to 57% by EIA. However, for every sample tested, absorption with homologous virus removed more activity than did heterologous virus. An 89-kDa protein was identified as an HHV-7-specific serologic marker by IB. Activity to this protein was not removed by absorption with HHV-6 antigen. Of the three assays, the EIA was the most sensitive (94%), while the IB was the most specific (94%). Approximately 80% of specimens collected from German adults and children older than 2 years were positive for HHV-7 antibodies by these assays.
机译：为了检测人血清中的人类疱疹病毒7（HHV-7）抗体，开发了一种酶联免疫测定（EIA），免疫印迹测定（IB）和间接免疫荧光测定。使用HHV-7和人类疱疹病毒6（HHV-6）抗原与EIA或IFA进行交叉吸收研究表明，大多数人血清包含交叉反应的HHV-6和HHV-7抗体，并且交叉反应的程度因个体而异血清标本。通过EIA对异源病毒的吸收来抑制同源抗体的活性为0至57％。但是，对于每个测试样品，与异源病毒相比，同源病毒的吸收去除了更多的活性。 IB将89kDa蛋白鉴定为HHV-7特异性血清学标志物。未被HHV-6抗原吸收而不能除去对该蛋白质的活性。在这三种测定中，EIA最敏感（94％），而IB最特异性（94％）。通过这些测定，从德国成年人和2岁以上儿童中收集的标本中约有80％对HHV-7抗体呈阳性。

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