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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Serological responses to human papillomavirus type 6 and 16 virus-like particles in patients with cervical neoplastic lesions.
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Serological responses to human papillomavirus type 6 and 16 virus-like particles in patients with cervical neoplastic lesions.

机译：宫颈肿瘤性病变患者对6型和16型人乳头瘤病毒的血清学反应。

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Serum samples from 36 cervical carcinoma patients, 33 patients with high-grade squamous intraepithelial lesions, and 31 cytologically normal women were tested by enzyme-linked immunosorbent assay (ELISA) using human papilloma virus type 6 (HPV 6) and HPV 16 virus-like particles as antigens. Forty serum specimens from 1-year-old children were used to assign cutoff points. When serum samples from the subjects infected with HPV 16 were tested in an HPV 16 ELISA detecting immunoglobulin A (IgA), IgG, and IgM binding, 61% showed IgA, 44% showed IgG, and 39% showed IgM reactivity. Of HPV 6- or 11- or HPV 18-infected subjects. fewer than 17% showed IgA or IgG responses and 33% showed IgM reactivity. In contrast, 13% showed IgA, 10% showed IgG, and 16% showed IgM reactivity in the HPV DNA-negative controls. The results suggest that the IgA and IgG responses are HPV 16 specific and the IgM response is cross-reactive to different HPV types. On the other hand, the serological responses to HPV 6 did not differ in the patient and control groups. The percentages of patients positive for both IgA and IgG antibodies were significantly higher in the groups with high-grade squamous intraepithelial lesions (12% [4 of 33]; P = 0.04) and cancer (17% [6 of 36]; P = 0.02) than in the healty women (0% [0 of 31]), and the percentages for either IgA or IgG were higher for the cancer group (47% [17 of 36]; P = 0.01) than in the normal group (19% [6 of 31]). Most sera positive for IgA and IgG in the patient groups showed higher titers than those in the normal group. All these results suggest that high IgA and IgG responses are good indicators for estimating HPV 16 infection.
机译：使用6型人乳头瘤病毒（HPV 6）和类HPV 16病毒样通过酶联免疫吸附法（ELISA）检测了36例宫颈癌患者，33例高度鳞状上皮内病变患者和31例细胞学正常女性的血清样本颗粒作为抗原。一岁儿童的40份血清标本被用来作为临界点。当在HPV 16 ELISA中检测来自感染HPV 16的受试者的血清样品后，检测到免疫球蛋白A（IgA），IgG和IgM的结合情况，其中61％显示IgA，44％显示IgG，39％显示IgM反应性。 HPV 6或11或HPV 18感染的受试者。少于17％的人表现出IgA或IgG反应，而33％的人表现出IgM反应性。相反，在HPV DNA阴性对照中，有13％显示IgA，10％显示IgG和16％显示IgM反应性。结果表明，IgA和IgG应答是HPV 16特异性的，而IgM应答对不同的HPV类型具有交叉反应性。另一方面，在患者和对照组中，对HPV 6的血清学反应没有差异。在高度鳞状上皮内病变组（12％[4 of 33]; P = 0.04）和癌症组（17％[6 of 36]）中，IgA和IgG抗体均为阳性的患者百分比明显更高。 （0.02％）比健康女性（0％[0 of 31]），癌症组的IgA或IgG百分比更高（47％[17 of 36]; P = 0.01），高于正常组（ 19％[31 of 6]）。患者组中大多数IgA和IgG阳性血清的滴度均高于正常组。所有这些结果表明，高IgA和IgG应答是评估HPV 16感染的良好指标。

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