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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Validation of an In-House Assay for Cytomegalovirus Immunoglobulin G (CMV IgG) Avidity and Relationship of Avidity to CMV IgM Levels
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Validation of an In-House Assay for Cytomegalovirus Immunoglobulin G (CMV IgG) Avidity and Relationship of Avidity to CMV IgM Levels

机译：对巨细胞病毒免疫球蛋白G（CMV IgG）亲和力及其与CMV IgM水平的关系的内部分析的验证

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Measurement of cytomegalovirus (CMV)-specific immunoglobulin G (IgG) avidity has proven to be a powerful tool for distinguishing primary from nonprimary CMV infection. An in-house enzyme-linked immunosorbent assay (ELISA) for measuring CMV IgG avidity was validated using 84 sera from pregnant women who had recently seroconverted following primary CMV infection and 74 sera from individuals with past CMV infection (IgG-positive and IgM-negative profile). Of the 84 sera from pregnant women, 73 sera were collected within 120 days of the last IgG-negative sample, and 72 of these 73 sera (99%) exhibited an avidity index (AI) of <50%. In contrast, 71 of 74 (96%) sera from individuals with past CMV infection exhibited CMV AI values of >60%. Thus, low avidity in the in-house ELISA was defined as an AI of ?50%, whereas high avidity was defined as an AI of ?60%. In additional studies, the relationship between CMV IgG avidity and CMV IgM levels was examined using 64 CMV IgG-positive sera (time since seroconversion unknown) exhibiting equivocal or positive results in a CMV IgM capture ELISA (Diamedix). Of these 64 sera, 29 exhibited IgM index values of ?3.0, and 27 of these 29 (93%) exhibited low IgG avidity. A similar trend was observed when a subset of these 64 sera (n = 48) was tested in another CMV IgM capture ELISA (Trinity); of 18 sera with IgM index values of ?3.0, 17 (94%) exhibited low IgG avidity. These findings demonstrate the validity of an in-house ELISA for CMV IgG avidity and further show that strong reactivity of CMV IgG-positive sera in either of two CMV IgM capture assays is a reliable indicator of low CMV IgG avidity, and thus, recent CMV infection.
机译：巨细胞病毒（CMV）特异性免疫球蛋白G（IgG）亲和力的测量已被证明是区分原发性和非原发性CMV感染的有力工具。使用来自初次CMV感染后最近发生血清转化的孕妇的84份血清和来自过去CMV感染的个体的74份血清（IgG阳性和IgM阴性）进行的内部酶联免疫吸附测定（ELISA）用于验证CMV IgG亲和力个人资料）。在孕妇的84份血清中，有73份血清是在最后一次IgG阴性样本的120天内收集的，这73份血清中的72份（99％）显示出的亲和力指数（AI）<50％。相比之下，过去感染过CMV的个体的74份血清中有71份（96％）的CMV AI值> 60％。因此，内部ELISA的低亲和力定义为AI约为50％，而高亲和力的AI定义为AI60％。在其他研究中，使用64个CMV IgG阳性血清（自血清转化以来的时间未知）检查了CMV IgG亲和力与CMV IgM水平之间的关系，该结果在CMV IgM捕获ELISA（Diamedix）中表现出模棱两可或阳性结果。在这64个血清中，有29个的IgM指数值约为3.0，这29个中的27个（93％）的IgG亲和力低。当在另一个CMV IgM捕获ELISA（Trinity）中测试这64个血清的一部分（ n = 48）时，观察到类似的趋势; IgM指数值为3.0的18个血清中，有17个（94％）的IgG亲和力低。这些发现证明了针对CMV IgG亲和力的内部ELISA的有效性，并且进一步表明，在两种CMV IgM捕获试验中，CMV IgG阳性血清的强反应性是低CMV IgG亲和力的可靠指标，因此，最近的CMV感染。

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