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首页> 外文期刊>Acta Societatis Botanicorum Poloniae >Identification of novel and conserved microRNAs involved in fruit development and ripening in Fragaria vesca
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Identification of novel and conserved microRNAs involved in fruit development and ripening in Fragaria vesca

机译：鉴定与草莓属植物果实发育和成熟有关的新颖且保守的microRNA

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摘要

MicroRNAs (miRNAs) are class of noncoding RNAs that regulate gene expression at the post-transcriptional level, either by endonucleolytic cleavage or by translational inhibition. Strawberry is a popular worldwide fresh fruit and is believed to benefit human health. However, the function of miRNAs during this fruit development and ripening remains unknown and miRNAs for specific for these processes are expected to be discovered. In the study, we identified 218 conserved miRNAs and 87 novel miRNAs in Fragaria vesca. Expression profiling of miRNAs during fruit development and ripening was performed, and the expression of targets of the miRNAs was validated by qRT-PCR (quantitative reverse transcription polymerase chain reaction). This study provided data for further research on molecular mechanisms involved in fruit development and ripening.
机译：微小RNA（miRNA）是一类非编码RNA，可通过内切核酸酶切或翻译抑制作用在转录后水平调控基因表达。草莓是世界范围内流行的新鲜水果，被认为有益于人类健康。但是，在这种果实发育和成熟过程中，miRNA的功能仍然是未知的，并且有望发现这些过程特异的miRNA。在这项研究中，我们在草莓中鉴定了218个保守的miRNA和87个新的miRNA。进行了果实发育和成熟过程中miRNA的表达谱分析，并通过qRT-PCR（定量逆转录聚合酶链反应）验证了miRNA靶标的表达。该研究为进一步研究果实发育和成熟的分子机制提供了数据。

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