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首页> 外文期刊>Acta Societatis Botanicorum Poloniae >Tracking of intercalary DNA sequences integrated into tandem repeat arrays in rye Secale vavilovii
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Tracking of intercalary DNA sequences integrated into tandem repeat arrays in rye Secale vavilovii

机译：跟踪整合到黑麦Secale vavilovii中的串联重复序列的插入DNA序列

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The structure of repetitive sequences of the JNK block present in the pericentromeric region of the 2RL chromosome was studied in Secale vavilovii. Amplification of sequences present between the JNK sequences led to the identification of seven abnormal DNA fragments. Two of these fragments showed high similarity to the glutamate 5-kinase gene and putative alcohol dehydrogenase gene of trypanosomatid from the genus Leishmania, whose presence can be explained by horizontal gene transfer (HGT). Other fragments were similar to mitochondrial gene for ribosomal protein S4 in plants and to the glycoprotein (G) gene of the IHNV virus. Presumably, they are pseudogenes inserted into the JNK heterochromatin region. Within this region, also fragments similar to the rye repetitive sequence and chromosome 3B in wheat were found. There is no known mechanism that would explain how foreign sequences were inserted into the block region of tandem repetitive sequences of the JNK family.
机译：在Secale vavilovii中研究了存在于2RL染色体的着丝粒区域中的JNK嵌段的重复序列的结构。 JNK序列之间存在的序列的扩增导致鉴定了七个异常DNA片段。这些片段中的两个显示与来自利什曼原虫属的锥虫的谷氨酸5-激酶基因和推定的醇脱氢酶基因高度相似，其存在可以通过水平基因转移（HGT）来解释。其他片段类似于植物中核糖体蛋白S4的线粒体基因以及IHNV病毒的糖蛋白（G）基因。据推测，它们是插入JNK异染色质区域的假基因。在该区域内，还发现了类似于小麦中黑麦重复序列和3B染色体的片段。没有已知的机制可以解释如何将外源序列插入JNK家族的串联重复序列的区块区域。

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