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首页> 外文期刊>African Journal of Agricultural Research >In silico identification of putative expressed sequence tag (EST)-simple sequence repeats (SSRs) markers of resistance to Meloidogyne spp. in common bean
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In silico identification of putative expressed sequence tag (EST)-simple sequence repeats (SSRs) markers of resistance to Meloidogyne spp. in common bean

机译：在计算机上鉴定对Meloidogyne spp的抗性的推定表达序列标签（EST）-简单序列重复（SSR）标记。在普通豆

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Expressed sequences are important sources in the development of functional heterologous microsatellite markers in phylogenetic related groups, that is, soybean and commom bean. The objective of this work was to identify and characterize expressed sequence tag (EST)-simple sequence repeats (SSRs) in silico candidates of resistance to Meloidogyne spp. in common bean. Seven DNA sequences from soybean associated with genetic resistance have been identified and obtained in the NCBI database. Its homology in common bean genome was verified using the BLAST tool. The cellular processes involved were also checked using the Blast2GO program. The identification of microsatellite markers and design of the primer pairs was performed using the SSRLocator and Primer3 programs, respectively. The transferability rate of common beans the target sequences identified was 86%, demonstrating the power of success of this method. All the cellular processes involved in the original DNA sequences were verified from EST on beans, with E-value between 0 and 2.9×10-168. Fifteen EST-SSRs candidates for common bean resistance were identified, which have proved to be suitable for their amplification by PCR. The transferability analysis of ESTs related to resistance to Meloidogyne spp., especially among soybeans and common beans is efficient. Based on this study, 15 EST-SSRs candidates are available for validation and later use.
机译：表达的序列是系统发育相关群体中功能​​异源微卫星标记发展的重要来源，即大豆和普通豆。这项工作的目的是鉴定和鉴定对拟根结线虫菌种抗性的计算机候选表达序列标签（EST）-简单序列重复（SSR）。在普通豆中。已经从NCBI数据库中鉴定并获得了与遗传抗性相关的大豆的7条DNA序列。使用BLAST工具验证了其在普通豆基因组中的同源性。还使用Blast2GO程序检查了涉及的细胞过程。分别使用SSRLocator和Primer3程序进行微卫星标记的鉴定和引物对的设计。靶序列确定的普通豆的转移率为86％，证明了该方法的成功。原始DNA序列中涉及的所有细胞过程均通过EST在豆上进行了验证，E值介于0和2.9×10-168之间。鉴定出十五种普通豆抗性的EST-SSR候选物，已证明适合通过PCR扩增。与抗Meloidogyne spp。有关的ESTs的可转移​​性分析，尤其是在大豆和普通豆中，是有效的。根据这项研究，有15个EST-SSR候选物可供验证和以后使用。

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