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首页> 外文期刊>African Journal of Biotechnology >Co-extraction of high-quality RNA and DNA from rubber tree (Hevea brasiliensis)
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Co-extraction of high-quality RNA and DNA from rubber tree (Hevea brasiliensis)

机译：从橡胶树（巴西橡胶树）中共提取高质量的RNA和DNA

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High-quality nucleic acids are the basic requirement for performing genomic research. A reliable and efficient method was developed for co-extracting high-quality DNA and RNA from rubber tree (Hevea brasiliensis)?in this study. Polyethylene glycol (PEG) and cetyltrimethylammonium bromide (CTAB) extraction buffer with high concentrations of polyvinylpyrrolidone (PVP) and?b-mercaptoethanol was used in this study. The results show that 3.2% polyethylene glycol 8000 is the optimal concentration for successful separation of DNA and RNA. Spectrophotometric determination (A260/A280?and A260/A230?ratios), agarose electrophoresis analysis and reverse transcription (RT-PCR) of isolated nucleic acids indicate that high-quality DNA and RNA were extracted by this method. The general applicability of this method was also evaluated, and the results show that it was suitable for a variety of plants.
机译：高质量的核酸是进行基因组研究的基本要求。在本研究中，开发了一种可靠且有效的方法，用于从橡胶树（巴西橡胶树）中共提取高质量的DNA和RNA。本研究中使用了具有高浓度聚乙烯吡咯烷酮（PVP）和βb-巯基乙醇的聚乙二醇（PEG）和十六烷基三甲基溴化铵（CTAB）提取缓冲液。结果表明，3.2％的聚乙二醇8000是成功分离DNA和RNA的最佳浓度。分光光度法测定（A260 / A280和A260 / A230），琼脂糖电泳分析和逆转录（RT-PCR）分离出的核酸表明，该方法提取了高质量的DNA和RNA。还评估了该方法的一般适用性，结果表明该方法适用于多种植物。

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