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首页> 外文期刊>African Journal of Biotechnology >Molecular characterization of virus isolates from genus Potyvirus infecting Vigna subterranea in Burkina Faso
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Molecular characterization of virus isolates from genus Potyvirus infecting Vigna subterranea in Burkina Faso

机译：来自布吉纳法索的Vigna地下马铃薯病毒的分离株的分子特征

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Bambara groundnut (Vigna subterranea) is an African legume with a great nutritional, economic and social potential. However, one of the main constraints to this crop is viral diseases which reduced yields. Indeed, approximately 12 viruses have been reported to infect bambara groundnut. Among these, only four were reported from Burkina Faso, namely, cowpea aphid-borne mosaic virus (CABMV), bean common mosaic virus strain-blackeye cowpea mosaic (BCMV-BlCM), peanut mottle virus (PeMoV), and cowpea mottle virus (CPMoV). This study was carried out in order to identify and characterize the main viruses occurring in bambara groundnut from Burkina Faso using serological and molecular tests. 140 plants were sampled in the three agro-climatic zones of Burkina Faso. Both Potyvirus CABMV and BCMV-BlCM were identified in 8.57% of the samples when double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) test was used and 14.29% of the samples when reverse transcription-polymerase chain reaction (RT-PCR) test was used. Phylogenetic tree based on 476 nt in coat protein showed that all Burkina Faso CABMV isolates clustered together with Uganda isolate (KT726938). However, two groups were distinguished within these isolates. Burkina Faso BCMV-BlCM isolates are strengthly clustered with BCMV and BlCMV group. This study reports the first molecular characterization of CABMV and BCMV-BlCM infecting Bambara groundnut in Burkina Faso.
机译：班巴拉花生（Vigna subterranea）是一种非洲豆类，具有很大的营养，经济和社会潜力。但是，这种作物的主要限制因素之一是病毒性疾病，这降低了产量。实际上，据报道大约有12种病毒感染巴巴拉花生。其中，布基纳法索仅报告了四种four豆蚜虫传播的花叶病毒（CABMV），豆类普通花叶病毒株黑眼cow豆花叶病毒（BCMV-BlCM），花生斑驳病毒（PeMoV）和，豆斑驳病毒（ CPMoV）。进行这项研究是为了通过血清学和分子测试鉴定和鉴定来自布基纳法索的班巴拉花生的主要病毒。在布基纳法索的三个农业气候区采样了140株植物。使用双抗体夹心酶联免疫吸附法（DAS-ELISA）检测时，在8.57％的样品中鉴定出波多病毒CABMV和BCMV-BlCM，而使用逆转录聚合酶链反应（RT-PCR）时则在样品中鉴定出了14.29％测试被使用。基于外壳蛋白中476 nt的系统进化树显示，所有布基纳法索CABMV分离株均与乌干达分离株（KT726938）聚集在一起。但是，在这些分离物中分为两组。布基纳法索BCMV-B1CM分离株与BCMV和BlCMV组强力簇集。这项研究报告了感染布基纳法索的班巴拉花生的CABMV和BCMV-BlCM的第一个分子特征。

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