Production of phytate-hydrolyzing enzymes by thermophilic moulds

Bijender Singh; T. Satyanarayana

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Production of phytate-hydrolyzing enzymes by thermophilic moulds

机译：通过嗜热霉菌生产肌醇六磷酸水解酶

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138 isolates of thermophilic/thermotolerant moulds were isolated from soil, straw and compost samples, collected from various regions of India. Among the thermophilic fungal isolates screened for the secretion of phytase (phytate-hydrolyzing enzyme),Sporotrichum thermophile?BJTLR50 produced a very high enzyme titre at pH 5.0, 45°C and 250 rpm in 5 days with an inoculum level of 1 × 107?spores per 50 ml medium prepared from 6 days old culture. Glucose and ammonium sulphate supported higher phytase production than other carbon and nitrogen sources. The phytase of?S. thermophile?was optimally active at pH 5.0 and 60°C. An overall 2-fold improvement in the phytase production was achieved due to optimization.

机译：从印度各个地区收集的土壤，稻草和堆肥样品中分离出138个嗜热/耐热霉菌菌株。在筛选出分泌植酸酶（植酸水解酶）的嗜热真菌分离株中，嗜热孢子菌BJTLR50在5天的pH 5.0、45°C和250 rpm下产生了很高的酶滴度，接种量为1×107？从培养6天后的培养基中每50毫升培养基中提取孢子。葡萄糖和硫酸铵比其他碳源和氮源支持更高的植酸酶产量。 S.的植酸酶。嗜热菌在pH 5.0和60°C时具有最佳活性。由于优化，植酸酶产量总体提高了2倍。

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《African Journal of Biotechnology》 |2012年第59期|共11页
作者
Bijender Singh; T. Satyanarayana;
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中图分类生物工程学（生物技术）;
关键词
Phytic acidphytasethermophilic mouldsSporotrichum thermophileHumicola lanuginosasubmerged fermentationoptimization;

机译：植酸植酸菌的霉菌嗜热孢子菌Humicola lanuginosa浸没发酵的优化;

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Production of phytate-hydrolyzing enzymes by thermophilic moulds

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