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首页> 外文期刊>African Journal of Biotechnology >Simple sequence repeat (SSR) and inter simple sequence repeat (ISSR) analyses of genetic diversity in tissue culture regenerated plants of cotton
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Simple sequence repeat (SSR) and inter simple sequence repeat (ISSR) analyses of genetic diversity in tissue culture regenerated plants of cotton

机译：简单序列重复（SSR）和简单序列间重复（ISSR）分析棉花组织培养再生植物的遗传多样性

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Cotton is one of the main economic crop plants of Iran cultivated under continuous artificial selection and cultivation which may lead to genetic erosion and possible loss of useful genetic loci resulting in vulnerability to pests and diseases. For this reason increasing and improving?the amount of genetic diversity in cotton germplasm through tissue culture is important. The present report considers genetic diversity induced in tissue culture regenerated plants of three cotton cultivars namely Mehr, Sindose and their hybrid Mehr X Sindose. Surface of seeds were disinfected with 70% ethanol for 2 min and then treated with 5% hypochlorite solution for 20 min. Finally, they were washed 3?to?4 times with sterile distilled water and inoculated aseptically onMurashige and Skoog (MS)?basal medium free hormones. Single nodes resulted from seedlings cultured as explants.?Inter simple sequence repeat (ISSR)?and?simple sequence repeat (SSR)?primers used produced different number of bands in the genotypes studied showing different levels of molecular polymorphisms in each cultivar. Some common and few specific ISSR/SSR loci were indentified while some bands were present in all the genotypes except one indicating genetic changes in them. Analysis of molecular variance?(AMOVA)?test showed significant difference (p < 0.05) for ISSR markers but not for SSR markers. Molecular trees obtained showed genetic variations among the regenerated plants of each cultivar due to tissue culture.
机译：棉花是伊朗在持续不断的人工选择和种植下种植的主要经济作物之一，可能导致遗传侵蚀和有用的遗传基因座可能丧失，从而导致对病虫害的脆弱性。因此，通过组织培养增加和改善棉花种质的遗传多样性非常重要。本报告考虑了三个棉花品种Mehr，Sindose及其杂种Mehr X Sindose在组织培养再生植物中诱导的遗传多样性。种子表面用70％乙醇消毒2分钟，然后用5％次氯酸盐溶液处理20分钟。最后，将它们用无菌蒸馏水洗涤3到4次，并在Murashige和Skoog（MS）？基础中性游离激素上无菌接种。单节点是由作为外植体培养的幼苗产生的。使用的内部简单序列重复（ISSR）和简单序列重复（SSR）引物在所研究的基因型中产生了不同数量的条带，显示了每个品种的分子多态性水平不同。确定了一些常见的和很少的特定ISSR / SSR基因座，而在所有基因型中都出现了一些条带，但一条带指示了它们的遗传变化。分子方差（AMOVA）检验的分析表明，ISSR标记差异显着（p <0.05），而SSR标记则无差异。由于组织培养，获得的分子树显示出每个品种的再生植物之间的遗传变异。

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