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首页> 外文期刊>African Journal of Biotechnology >Weaver gene 3UTR novel mutations: Associations with production traits and milk composition in dairy goat
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Weaver gene 3UTR novel mutations: Associations with production traits and milk composition in dairy goat

机译：韦弗基因3UTR新突变：与奶山羊生产性状和乳成分的关联

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Our?recent?report on a parallel increase in the milk yield of?weaver?gene mutation suggests that?weaver?gene is a candidate marker for quantitative traits in farm animals with respect to milk production traits. To further understand the effects ofweaver?gene variant on?fat, protein, solids-not-fat, lactose?and total solids percentage, two novel?single nucleotide polymorphisms (SNPs) located on the flanking 3'UTR region were investigated by?TaqI polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and?HhaI forced PCR-RFLP in a sample of 1,019 individuals from four Chinese indigenous goat breeds. In the?TaqI and?HhaI analyses, the frequencies of allele T2?and H2?in?Xinong Sannen dairy (XNSN dairy) and?Guanzhong dairy?(GZ dairy) populations were significantly greater than those of?Shanbei White Cashmere?(SBWC) and?Xinjiang White Cashmere(XJWC) goats, respectively. Relationships analyses between the two SNPs and milk performance traits and growth traits were performed. T2T2?animals had significantly greater milk yield, protein and solids-not-fat percentage than those with the T1T1genotype. Thus,?weaver/TaqI polymorphism appears to be a promising marker to improve milk production traits in goat.
机译：我们关于“ weaver”基因突变的产奶量平行增加的最新报告表明，“ weaver”基因相对于牛奶生产性状是家畜定量性状的候选标记。为了进一步了解weaver基因变异对脂肪，蛋白质，非脂肪固体，乳糖和总固体百分比的影响，通过TaqI研究了位于侧翼3'UTR区域的两个新颖的单核苷酸多态性（SNP）。来自四个中国本土山羊品种的1,019个个体的样本中，聚合酶链反应限制片段长度多态性（PCR-RFLP）和？HhaI强制PCR-RFLP。在“ TaqI”和“ HhaI”分析中，“西农三嫩”奶牛（XNSN奶牛）和“关中奶牛”（GZ奶牛）的等位基因T2和H2的频率显着大于“陕北​​白绒”（SBWC）的）和新疆白羊绒（XJWC）山羊。进行了两个SNPs与牛奶生产性状和生长性状之间的关系分析。与具有T1T1基因型的动物相比，T2T2动物的牛奶产量，蛋白质和非脂肪固体百分比显着更高。因此，？weaver / TaqI多态性似乎是改善山羊产奶性状的有前途的标志。

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