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Development of species-specific primers for identifying Auricularia auricula-judae using intergenic spacer 1 (IGS1) sequences

机译:利用基因间隔子1(IGS1)序列开发特定物种的引物,以鉴定小木耳

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Auricularia auricula-judae,?an important edible and medicinal mushroom was first cultivated in China more than one thousand years ago. In this study, we developed a simple and rapid?polymerase chain reaction (PCR)-based diagnostic tool for identifying?A. auricula-judae?from other morphologically similar?Auricularia?species.Based on the sequence analysis of intergenic spacer 1 (IGS1) in genus?Auricularia, two species-specific primer combinations were designed from IGS1 sequence of?A. auricula-judae, both of them can generate DNA fragments of different size unique toA. auricula-judae. The validity of this two primer combinations were confirmed by correctly amplifying species-specific fragments from genomic DNA of another?32?A. auricula-judae?strains. To our knowledge, this was the first study on development of species-specific primers via IGS1 sequence in jelly fungi and also the first application of IGS1 sequence in genus?Auricularia.
机译:重要的食用和药用蘑菇金耳木(Auricularia auricula-judae)于一千多年前首次在中国栽培。在这项研究中,我们开发了一种基于简单,快速的聚合酶链反应(PCR)的诊断工具来识别A。根据其他形态相似的黑木耳(Auricularia)种的黑木耳(Auricula-judae)。基于黑木耳属(Ausicularia)的基因间隔基1(IGS1)的序列分析,从βA的IGS1序列设计了两种物种特异性引物组合。极光金刚鹦鹉,它们都可以产生A独有的不同大小的DNA片段。极光judae。这两种引物组合的正确性是通过从另一个Δ32ΔA的基因组DNA中正确扩增物种特异性片段来证实的。极光judae?菌株。据我们所知,这是通过IGS1序列在果冻真菌中开发物种特异性引物的第一项研究,也是IGS1序列在黑穗菌属中的首次应用。

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