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首页> 外文期刊>African Journal of Biotechnology >Growth of Enterococcus durans E204 producing bacteriocin-like substance in MRS Broth: Description of the growth and quantification of the bacteriocin-like substance
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Growth of Enterococcus durans E204 producing bacteriocin-like substance in MRS Broth: Description of the growth and quantification of the bacteriocin-like substance

机译：在MRS肉汤中产生杜兰肠球菌E204的细菌素样物质的生长：细菌素样物质的生长和定量的描述

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摘要

Bacteriocin-like substance E204 is an antimicrobial compound produced byEnterococcus durans?E204 isolated from camel milk of Morocco that shows a broad spectrum of inhibitory activity against taxonomically related microorganisms. It is sensitive to digestive proteases. In the first study, de Man, Regosa and Sharpe (MRS) broth was inoculated by E204 strain, incubated for 30°C at 200 rpm and monitored by checking dry cell weight, nutrients consumption, lactic acid and bacteriocin-like productions. The maximum biomass (2.3 g/l) and antimicrobial activity (32 AUml-1) were obtained at 12 h of incubation. No increase in the production of bacteriocin-like was recorded after the exponential phase. In the other hand, the quantification of the antimicrobial activity was carried out by a photometric assay on culture tubes based on the determination of the ID50 dose causing 50% growth inhibition of Enterococcus faecium 410 CECT in 6 h of incubation. The highest bacteriocin-like titre (279.71 BUml-1) was obtained at acidic pH (3.5) and at 70°C after 10 min of incubation.
机译：类细菌素E204是从摩洛哥骆驼奶中分离得到的杜伦肠球菌E204产生的抗菌化合物，对分类学相关的微生物表现出广泛的抑制活性。它对消化蛋白酶敏感。在第一个研究中，用E204菌株接种了De Man，Regosa和Sharpe（MRS）肉汤，以200 rpm的温度孵育30°C，并通过检查干细胞重量，营养消耗，乳酸和细菌素样产物进行监测。在孵育12小时后获得最大生物量（2.3 g / l）和抗菌活性（32 AUml-1）。在指数期之后，没有记录到细菌素样产生的增加。另一方面，通过对培养管进行光度测定来进行抗微生物活性的定量，其方法是根据ID50剂量的确定来确定ID50剂量，该剂量在培养6小时后会抑制粪肠球菌410 CECT的50％生长。孵育10分钟后，在酸性pH（3.5）和70°C下获得最高的细菌素样滴度（279.71 BUml-1）。

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