• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Molecular detection of TasA gene in endophytic Bacillus species and characterization of the gene in Bacillus amyloliquefaciens
【24h】

Molecular detection of TasA gene in endophytic Bacillus species and characterization of the gene in Bacillus amyloliquefaciens

机译：内生芽孢杆菌中TasA基因的分子检测及解淀粉芽孢杆菌中该基因的表征

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

TasA, the gene which play an important role in bacteria development, physiology and bacteria biofilm formation in?Bacillus?species?was detected in the endophytic bacteria by?polymerase chain reaction?(PCR)?amplification. In ten?endophytic?Bacillus?strains tested,?TasA?gene was readily detected in?Bacillus?amyloliquefaciens?PEBA20 and 7 strains of?Bacillus?subtilis, whereas?Bacillus?megaterium?and?Bacillus?cereus?did not exhibit detectable gene of?TasA. The?TasA?gene cloned from?B. amyloliquefaciensconsists of the intact open reading frame (ORF) of 786 base pairs encoding a protein of 261 amino acid residues including a conserved signal peptide consisting of 27 amino acid residues. Sequences analyses revealed?TasA?is highly?conserved; however,?there is certain difference in the nucleotide sequence and deduced amino acid sequence among the genes harbored in?B. amyloliquefaciens?and?B. subtilis, implying the possible distinction of structure and function of the protein in different bacteria species. This study may be beneficial for the understanding of the genes harbored in?Bacillus?species in nature and may lead to further study on the gene function.
机译：通过“聚合酶链反应”（PCR）扩增，在内生细菌中检测到TasA，该基因在“芽孢杆菌”种中对细菌的发育，生理和细菌生物膜的形成起着重要作用。在测试的十个内生芽孢杆菌菌株中，很容易在解淀粉芽孢杆菌PEBA20和7株枯草芽孢杆菌中检测到TasA基因，而巨大芽孢杆菌和蜡状芽孢杆菌未显示可检测的基因。的？从？B克隆的？TasA？基因。淀粉寡糖淀粉酶由786个碱基对的完整开放阅读框（ORF）组成，其编码261个氨基酸残基的蛋白质，包括由27个氨基酸残基组成的保守信号肽。序列分析显示“ TasA”是高度保守的。但是，B中携带的基因之间的核苷酸序列和推导的氨基酸序列有一定的差异。淀粉淀粉酶和B.枯草芽孢杆菌，暗示了在不同细菌物种中蛋白质结构和功能的可能区别。这项研究可能有助于了解自然界中芽孢杆菌种中所携带的基因，并可能导致对该基因功能的进一步研究。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号