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Genetic analysis of baculovirus resistance in lepidopteran model insect Bombyx mori L.

机译:鳞翅目模型昆虫家蚕抗杆状病毒的遗传分析。

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In order to clarify the resistant mechanism of BmNPV in silkworm, and from negative to prove agricultural pest inheritance of virus resistance, in this study, we used the highly resistant strain NB and susceptible strain 306 as the material through the method of classical genetics experiment, and proved that the baculovirus resistance in silkworm is controlled by a pair of autosomal dominant major gene. At the same time, we used random amplification of polymorphic DNA (RAPD) random primers to screen a molecular marker which are in high linkage with the resistant trait. Validity of the molecular marker was proved in BC1,?F2?populations, which further demonstrated that the baculovirus resistance in silkworm is controlled by a pair of autosomal dominant major gene. This can provide an effective research basis for the emergence of baculovirus resistance in pest and its resistant mechanism.
机译:为了阐明家蚕BmNPV的抗性机理,并从阴性结果证明农作物有害生物对病毒具有抗性遗传,本研究采用经典遗传实验方法,以高抗性菌株NB和易感菌株306为材料,并证明家蚕杆状病毒的抗性是由一对常染色体显性主基因控制的。同时,我们使用多态性DNA(RAPD)随机引物的随机扩增来筛选与抗性性状高度相关的分子标记。该分子标记的有效性在BC1,ΔF2α群体中得到证实,这进一步证明了家蚕中的杆状病毒抗性是由一对常染色体显性主要基因控制的。这可以为杆状病毒在害虫中的耐药性及其耐药机制的产生提供有效的研究依据。

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