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首页> 外文期刊>African Journal of Biotechnology >Construction of genetic map in barley using sequence-related amplified polymorphism markers, a new molecular marker technique
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Construction of genetic map in barley using sequence-related amplified polymorphism markers, a new molecular marker technique

机译：利用序列相关的扩增多态性标记物，一种新的分子标记技术构建大麦遗传图谱

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Sequence-related amplified polymorphism (SRAP) markers, a novel polymerase chain reaction (PCR)-based molecular marker technique, were successfully applied in map construction, cultivar identification, diversity evaluation, comparative genomics and gene location of different plant species. The molecular genetic map of SRAP markers in Steptoe / Morex doubled haploid (DH) population was constructed in this study, using 216 SRAP markers and 312 simple sequence repeat (SSR) markers. Overall, 21 of the 216 SRAP markers generated 78 polymorphic loci, and 98 of 312 SSR markers produced 107 polymorphic loci. Among the 185 loci, 175 loci (70 SRAP loci and 105 SSR loci) were assigned to nine linkage groups. The map covered 1475 cM with a mean density of 8.7 cM per locus. In total, 33 of all the loci (17.84%) showed significant segregation distortion. Moreover, 23 of the 33 loci (69.7%) skewed towards the parent Steptoe, whereas the remaining loci (21.3%) deviated towards the parent Morex and some of these distorted loci tended to cluster at the end of linkage groups, while others were dispersed on linkage groups in a decentralized fashion. The three putative segregation distortion regions (SDRs) were detected on chromosomes 2H, 4H and 5H, respectively. This linkage map?indicates its importance?in quantitative trait loci (QTLs) mapping, marker-assisted selection (MAS) and integrative analysis for further genetic studies with other linkage maps in barley.
机译：序列相关的扩增多态性（SRAP）标记，一种基于聚合酶链反应（PCR）的新型分子标记技术，已成功应用于不同植物物种的图谱构建，品种鉴定，多样性评估，比较基因组学和基因定位。在这项研究中，使用216个SRAP标记和312个简单序列重复（SSR）标记构建了Steptoe / Morex双单倍体（DH）群体中SRAP标记的分子遗传图谱。总体而言，在216个SRAP标记中有21个产生了78个多态位点，在312个SSR标记中有98个产生了107个多态位点。在185个基因座中，将175个基因座（70个SRAP基因座和105个SSR基因座）分配给了9个连锁组。该图覆盖了1475 cM，每个位点的平均密度为8.7 cM。总共，所有基因座中的33个（占17.84％）显示出明显的偏析变形。此外，在33个基因座中，有23个（69.7％）偏向父本Steptoe，而其余基因座（21.3％）向母本Morex偏斜，其中一些失真的基因座倾向于在连锁群的末端聚集，而另一些则分散以分散的方式讨论联系小组。分别在2H，4H和5H染色体上检测到三个假定的分离畸变区（SDR）。该连锁图表明了它在大麦性状的定量特征位点（QTL）作图，标记辅助选择（MAS）和综合分析方面的重要性。

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