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首页> 外文期刊>African Journal of Biotechnology >Genetic diversity of rice (Oryza sativa) germplasm from six countries using simple sequence repeats markers
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Genetic diversity of rice (Oryza sativa) germplasm from six countries using simple sequence repeats markers

机译：使用简单的序列重复标记，来自六个国家的水稻（Oryza sativa）种质的遗传多样性

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摘要

This experiment was carried out to determine the genotypic variation among rice (Oryza sativa) accessions using simple sequence repeats (SSR) markers. In the present study, a total of 12 SSR markers were used across 87 rice accessions from six countries. NTSYS-pc and PowerMarker software were used for data analysis. Six primers out of these 12 primers showed DNA amplification and polymorphism among the 87 rice accessions. The number of alleles detected by these six primers ranged from 2 to 9 with an average of 6.83 while polymorphism information content (PIC) ranged from 0.34 to 0.79 with an average of 0.55. The unweighted pair group method with arithmetic averages (UPGMA) cluster dendrogram generated based on the six SSR markers grouped the accessions into 4 clusters with 41% similarity coefficient. Accessions from these four clusters have late maturity, green basal leaf sheath colour, no awn and fewer tillers, respectively. This experiment has proven that even a small number of SSR markers are effective in assessing genetic diversity in rice. The genetic diversity revealed by the SSR markers in this study would be very important to select potentially good genotypes for future rice improvement programmes.
机译：使用简单序列重复（SSR）标记物进行了该实验以确定水稻（Oryza sativa）种质之间的基因型变异。在本研究中，来自六个国家的87个水稻品种共使用了12个SSR标记。使用NTSYS-pc和PowerMarker软件进行数据分析。在这12个引物中，有6个引物显示出87个水稻品种中的DNA扩增和多态性。这六个引物检测到的等位基因数量在2到9之间，平均为6.83，而多态性信息含量（PIC）在0.34到0.79之间，平均为0.55。基于六个SSR标记生成的具有算术平均值（UPGMA）聚类树状图的非加权对分组方法将种质分为4个具有41％相似系数的聚类。来自这四个簇的种质分别具有较晚成熟，基叶鞘绿色，无芒和分较少的特征。该实验已经证明，即使是少数SSR标记也可以有效地评估水稻的遗传多样性。在这项研究中，由SSR标记揭示的遗传多样性对于选择潜在的良好基因型用于未来的水稻改良计划将非常重要。

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