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首页> 外文期刊>African Journal of Biotechnology >Antifungal activities of basil (Ocimum basilicum L.) extract on Fusarium species
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Antifungal activities of basil (Ocimum basilicum L.) extract on Fusarium species

机译：罗勒（Ocimum basilicum L.）提取物对镰刀菌属物种的抗真菌活性

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The basil extract composition was determined by the GC-MS method and 38 different components were identified. The major components of the basil extract were estragol (86.72%),?trans-α-bergamotene (2.91%), eucalyptol (2.67%),?trans-ocimene (1.04%), linalool (0.72%), methyl-eugenol (0.71%), etc. The antifungal potential of the basil extract was tested against?Fusarium?oxysporum,?F. proliferatum,?F. subglutinans,?and?F. verticillioides?isolated from cakes, using the agar plate method. Extract concentrations of 0.35 and 0.70% (v/v) significantly inhibited the growth of?F. proliferatum?(33.37 and 44.30%, respectively) and?F. subglutinans?(24.74 and 29.27%, respectively) whereas other investigatedFusarium?species exhibited much lower sensitivity. The basil extract completely inhibited the growth of investigated?Fusarium?spp. at the concentration of 1.50% (v/v). Higher concentrations (0.35 and 0.70% (v/v)) reduced growth of aerial mycelium in all tested species. Strong medium pigmentation in the case of?F. proliferatum?and?F.?verticillioides?was observed. The microscopic examination of the samples confirmed the presence of hyphae deformations with a frequent occurrence of fragmentations, thickenings and diminished sporulation. In addition to the basic,?sensory, role the extract of basil has in the food product, it exerted significant antifungal properties, depending on its concentration.
机译：罗勒提取物的组成通过GC-MS方法确定，并鉴定出38种不同的成分。罗勒提取物的主要成分为雌二醇（86.72％），反式-α-佛手柑（2.91％），桉树油（2.67％），反式-前烯（1.04％），芳樟醇（0.72％），甲基丁香酚（罗勒提取物的抗真菌潜力已针对？Fusarium？oxysporum，？F进行了测试。增生？次胶质糖和？使用琼脂平板法从蛋糕中分离出丝状拟南芥。提取物浓度为0.35和0.70％（v / v）会显着抑制ΔF的生长。 proliferatum？（分别为33.37和44.30％）和F。亚谷蛋白亚种（分别为24.74和29.27％），而其他调查的F种则表现出低得多的敏感性。罗勒提取物完全抑制了所研究的F镰刀菌的生长。浓度为1.50％（v / v）。更高的浓度（0.35和0.70％（v / v））降低了所有测试物种中气生菌丝的生长。 ？F时，中等色素沉着。观察到增生和F.verticillioides。样品的显微镜检查证实了菌丝变形的存在，并频繁发生碎裂，增厚和孢子形成减少。罗勒提取物除了在食品中具有基本的“感官”作用外，还根据其浓度发挥了重要的抗真菌性能。

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