首页> 外文期刊>African Journal of Biotechnology >Essential oil composition of different fractions of Piper guineense Schumach. et Thonn from Cameroon using gas chromatography-mass spectrometry and their insecticidal effect on Sitophilus oryzae (L.)
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Essential oil composition of different fractions of Piper guineense Schumach. et Thonn from Cameroon using gas chromatography-mass spectrometry and their insecticidal effect on Sitophilus oryzae (L.)

机译:Piper guineense Schumach不同馏分的精油成分。气相色谱-质谱联用技术从喀麦隆获得的三溴甲烷等离子及其对米果Sitophilus oryzae(L.)的杀虫作用

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Essential oil fractions from dried seed powder of Piper guineense were analyzed by gas chromatography-mass spectrometry (GC-MS) and evaluated for their insecticidal effects on Sitophilus oryzae L. The GC-MS analysis showed quantitative and qualitative differences between the oil fractions. Chromatographic results revealed chemical constituents like eugenol, piperanol, pinene, carene, copaene with insecticidal properties. New chemotypes were seen in the different fractions. Instead of β-caryophyllene reported in literature, α-caryophyllene was found in all the different fractions. Caryophyllene oxide, an oxygen-containing sesquiterpene was present in all fractions except n-hexane. In addition to α-phellandrene present in all, β-phellandrene, a monoterpene hydrocarbon was found in the n-Hexane fraction. Contact toxicity on wheat grains showed that all fractions caused significant (P < 0.001) mortality of the weevils. The oil fractions also showed variable contact toxicity on impregnated filter paper. All doses of the n-hexane fraction were very toxic to the test insect than the control, causing 100% mortality after five days of exposure. All the fractions produced a strong repellent activity against the test insect. These results suggest that P. guineense has potentials for development as an organic insecticide against S. oryzae and other pests of stored grains.
机译:通过气相色谱-质谱法(GC-MS)分析了Piper guineense干燥种子粉中的精油馏分,并评估了其对米曲霉的杀虫效果。GC-MS分析表明,这些油馏分之间存在定量和定性差异。色谱结果表明,丁香酚,胡椒醇,pin烯,carene,copaene等化学成分具有杀虫性能。在不同部分中观察到新的化学型。代替文献中报道的β-石竹烯,在所有不同部分中都发现了α-石竹烯。除了正己烷外,所有馏分中都含有石蜡烯氧化物,一种含氧的倍半萜。除了全部存在的α-水芹烯,β-水芹烯之外,在正己烷馏分中还发现了单萜烃。对小麦籽粒的接触毒性表明,所有部分均引起象鼻虫的显着(P <0.001)死亡率。油馏分在浸渍滤纸上也显示出不同的接触毒性。所有剂量的正己烷馏分对受试昆虫的毒性均比对照高,暴露五天后会导致100%的死亡率。所有的级分均对测试昆虫产生强烈的驱避活性。这些结果表明,Guineense作为有机杀虫剂有潜力开发,可以对抗米曲霉和储存谷物的其他害虫。

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