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首页> 外文期刊>African Journal of Biotechnology >Phenylalanine ammonia-lyase (PAL) gene activity in response to proline and tyrosine in rosemary callus culture
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Phenylalanine ammonia-lyase (PAL) gene activity in response to proline and tyrosine in rosemary callus culture

机译：迷迭香愈伤组织培养物中苯丙氨酸解氨酶（PAL）基因活性对脯氨酸和酪氨酸的响应

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Phenylalanine ammonia-lyase (PAL) catalyzes the biosynthesis of rosmarinic acid (RA), tyrosine and phenylalanine are the precursors of RA, while proline drives metabolite precursors toward Shikimate and phenylpropanoid pathway ending with the production of RA. The aim of this study was to investigate the PAL gene activity in the callus of rosemary (Rosmarinus officinalis) due to exogenous application of L-proline and L-tyrosine. Four different concentrations of L-proline and L-tyrosine (0, 4, 5 and 6 mM, and 0, 0.4, 0.6 and 0.8 gm/L) respectively, were added to the basal Murashige and Skoog (MS) medium. The expression of the PAL gene was investigated and compared with the callus RA production. It was found that RA production increased significantly at low proline application (4 mM), on the other hand, tyrosine application at low concentrations had no effect on RA accumulation, while high tyrosine concentration (0.8 g/L) increased RA accumulation. When comparing PAL gene activity and RA production in callus tissues, it was found that they were correlated. Proline application alone at 4 mM or tyrosine alone at 0.8 g/L enhanced PAL gene activity, and also combining both proline at 4 mM and tyrosine at 0.8 g/L enhanced PAL gene activity and produced the highest RA accumulation in callus tissues [0.047 mg/g freash weight (fw)].
机译：苯丙氨酸氨裂合酶（PAL）催化迷迭香酸（RA）的生物合成，酪氨酸和苯丙氨酸是RA的前体，而脯氨酸则将代谢物前体推向Shi草酸和苯丙氨酸途径，最终以RA的产生为终点。这项研究的目的是调查由于外源应用L-脯氨酸和L-酪氨酸而引起的迷迭香（Rosmarinus officinalis）愈伤组织中PAL基因的活性。将四种不同浓度的L-脯氨酸和L-酪氨酸（分别为0、4、5和6 mM，以及0、0.4、0.6和0.8 gm / L）添加到基础Murashige和Skoog（MS）培养基中。研究了PAL基因的表达并将其与愈伤组织RA的产生进行了比较。发现在脯氨酸低施用（4 mM）时RA产量显着增加，另一方面，低浓度酪氨酸施用对RA积累没有影响，而高酪氨酸浓度（0.8 g / L）则增加RA积累。比较愈伤组织中的PAL基因活性和RA产生时，发现它们之间是相关的。单独以4 mM施用脯氨酸或以0.8 g / L单独施用酪氨酸可增强PAL基因活性，同时将4 mM脯氨酸和以0.8 g / L酪氨酸施用均可增强PAL基因活性，并在愈伤组织中产生最高的RA积累[0.047 mg / g freash重量（fw）]。

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