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Enzyme activity in bioregulator-treated tomato (Solanum lycopersicon) genotypes

机译:生物调节剂处理的番茄(Solanum lycopersicon)基因型中的酶活性

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The challenges of using exogenous plant bioregulators to manipulate plant responses by researchers and producers are increasing rapidly. In previous studies, we have demonstrated that 100 mg/l concentration of the bioregulators; indole-acetic acid (IAA), indole butyric acid (IBA) and naphthalene acetic acid (NAA) is suitable for tomato growth in the field. In this work, spectrophotometric analysis was conducted to determine the level of enzymes that could affect the keeping quality of the fruits harvested at the orange-red ripe stage following pre-sowing seed treatments with 100 mg/l solutions of the bioregulators. The NHLy 11, NHLy 12, NHLy 13, NHLy 15 and NHLy 16 genotypes were used and the activities of lipoxygenase (LOX), catalase, peroxidase, alcohol dehydrogenase and acid phosphatase determined. All treated genotypes showed high LOX activity with a range of 0.06 - 1.07?mmol.abs.units (A234). The NAA-treated NHLy13 genotype had complete loss of catalase activity after 210 s. Peroxidase activity was higher in NAA-treated genotypes?with values between 0.07 and 0.62 abs. units/g (A430) while alcohol dehydrogenase activity suffered a decline in all IBA-treated genotypes. IAA gave consistently higher acid phosphatase activity than IBA and NAA in all the test genotypes. These findings have important implications on the biochemical changes associated with the fruits during storage and processing.
机译:研究人员和生产者使用外源植物生物调节剂操纵植物反应的挑战正在迅速增加。在先前的研究中,我们已经证明了100 mg / l的生物调节剂浓度。吲哚乙酸(IAA),吲哚丁酸(IBA)和萘乙酸(NAA)适用于该领域的番茄生长。在这项工作中,进行了分光光度分析以确定可能影响橙红色成熟阶段收获的水果的保鲜质量的酶水平,这些种子在用100 mg / l的生物调节剂溶液进行预播种之后。使用NHLy 11,NHLy 12,NHLy 13,NHLy 15和NHLy 16基因型,并测定脂氧合酶(LOX),过氧化氢酶,过氧化物酶,醇脱氢酶和酸性磷酸酶的活性。所有处理过的基因型均表现出较高的LOX活性,范围为0.06-1.07?mmol.abs。单位(A234)。经NAA处理的NHLy13基因型在210 s后完全丧失了过氧化氢酶活性。在NAA处理的基因型中,过氧化物酶活性较高,其值在0.07至0.62 abs之间。单位/克(A430),而酒精脱氢酶活性在所有IBA处理的基因型中均下降。在所有测试基因型中,IAA始终提供比IBA和NAA高的酸性磷酸酶活性。这些发现对贮藏和加工过程中与水果相关的生化变化具有重要意义。

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