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首页> 外文期刊>African Journal of Biotechnology >Genomic affinity between Oryza sativa and Oryza brachyantha as revealed by in situ hybridization and chromosome pairing
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Genomic affinity between Oryza sativa and Oryza brachyantha as revealed by in situ hybridization and chromosome pairing

机译：通过原位杂交和染色体配对揭示水稻与水稻的基因组亲和力

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摘要

Genomic affinity between?Oryza sativa?(2n = 24 AA), and?Oryza brachyantha?(2n = 24 FF) was assessed by using three strategies: genomic?in situ?hybridization (GISH), meiotic chromosome pairing, pollen and spikelet sterility. The chromosome pairing was examined in pollen mother cells of?O. brachyantha,?O. sativa?and the hybrid between?O. sativa?and?O. brachyantha. The hybrid was highly sterile with no pollen stain ability. Both parents showed regular meiosis with normal chromosome pairing. The F1 hybrid exhibited limited chromosome pairing. On an average, 0-2 bivalents and 20-24 univalents were recorded at metaphase-1 and 0 - 1 univalent at diakinesis. The most frequent configuration was two bivalents and twenty univalent. The meiosis was highly irregular showing unequal distribution of chromosomes at anaphase, formation of multipolar bodies and variation in the cell cycle of both genomes. GISH revealed unequivocal discrimination of?O.?brachyanthachromosomes as appeared red from?O.?sativa?chromosomes that fluoresced yellow. No cross hybridization was examined between the labeled genomic DNA ofO.?brachyantha?and the chromosomes of?O.?sativa. Mitotic chromosomes of?O.brachyantha?and?O.?sativa, in the hybrid, were discriminated by GISH. High sterility in this hybrid could be due to abnormal meiosis and lack of pairing.
机译：利用以下三种策略评估了Oryza sativa（2n = 24 AA）和Brachyantha（2n = 24 FF）之间的基因组亲和力：基因组原位杂交（GISH），减数分裂染色体配对，花粉和小穗不育。在ΔO的花粉母细胞中检查了染色体配对。 Brachyantha，？O。苜蓿和'O'之间的杂种。苜蓿和？花chy。该杂种是高度无菌的，没有花粉染色能力。父母双方均表现出规则的染色体配对正常的减数分裂。 F1杂种表现出有限的染色体配对。平均而言，在中期诊断中记录了0-2个二价和20-24个单价，而在动态诊断中则记录了0-1个单价。最常见的构型是两个二价和二十个一价。减数分裂是高度不规则的，显示后期染色体分布不均，多极体的形成以及两个基因组细胞周期的变化。 GISH显示出对O.brachyantha染色体的明确辨别，从呈黄色发荧光的O.sativa-染色体呈现为红色。没有检测到O.?brachyantha？的标记基因组DNA和？O.？sativa的染色体之间的交叉杂交。用GISH鉴别杂种中的“ O.brachyantha”和“ O.sativa”的有丝分裂染色体。该杂种的高无菌性可能是由于异常减数分裂和缺乏配对。

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