• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Isolation and characterization of a novel Bacillus subtilis WD23 exhibiting laccase activity from forest soil
【24h】

Isolation and characterization of a novel Bacillus subtilis WD23 exhibiting laccase activity from forest soil

机译：新型漆酶活性枯草芽孢杆菌WD23的分离与鉴定

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The strain?Bacillus?sp. WD23 exhibiting laccase activity was screened from forest soil. The M9 medium containing Cu2+?was used for enriching and isolating bacterial strains capable of oxidizing syringaldazine. One isolated strain was identified asBacillus subtilis?WD23 based on the results of physiological and biochemical tests and 16S rDNA sequence analysis. The strain WD23 could grow at temperatures ranging from 20 to 55°C and showed optimum growth temperature and pH at 25°C and 7.0, respectively. The sporulation rate of the strain clearly correlated well with the laccase activity. The temperature half-life of the spore laccase was 2.5 h at 80°C and the pH half life time was 15 d at pH 9.0. Its spore laccase could decolorize 50 - 90% of Remazol brilliant blue R (RBBR), alizarin red, congo red, methyl orange and methyl violet, which suggests the potential application of spore laccase in dyestuff treatment.
机译：菌株是芽孢杆菌。从森林土壤中筛选出具有漆酶活性的WD23。含有Cu 2+的M9培养基被用于富集和分离能够氧化丁香醛嗪的细菌菌株。根据生理生化检测结果和16S rDNA序列分析，鉴定出一株枯草芽孢杆菌WD23。菌株WD23可以在20至55°C的温度范围内生长，并分别在25°C和7.0下显示最佳生长温度和pH。菌株的孢子形成率与漆酶活性明显相关。孢子漆酶的温度半衰期在80°C下为2.5 h，pH半衰期在pH 9.0下为15 d。它的孢子漆酶可以使Remazol艳蓝R（RBBR），茜素红，刚果红，甲基橙和甲基紫的50-90％脱色，这表明孢子漆酶在染料处理中的潜在应用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号