• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Characterization of spore laccase from Bacillus subtilis WD23 and its use in dye decolorization
【24h】

Characterization of spore laccase from Bacillus subtilis WD23 and its use in dye decolorization

机译：枯草芽孢杆菌WD23孢子漆酶的表征及其在染料脱色中的应用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A bacterial strain, designated as WD23, was isolated from the forest soil using M9 medium?supplemented with?0.4 mmol/L Cu2+. The spores from this strain showed laccase-like activity, oxidizing syringaldazine, 2,6-dimethoxyphenol and?2,2′-azino-bis (3-ethylbenzthiazoline-6-sulfonate acid) (ABTS). The strain was identified asBacillus subtilis?based on its morphological and physiological properties, and 16S rDNA sequence analysis. The?optimum pH and temperature for the spore-bound laccase were?6.8 and 60°C, respectively.?The temperature half-life of?the laccasewas 2.5 h at?80°C?and 68 h at?60°C.?It also showed high stabilities over a broad pH range. The pH half-life of?the laccase?was more than 6 months at pH 6.8. The sensitivities of the laccase to?organic solvents and inhibitors?were tested and it was found that laccase activity was strongly inhibited by methanol, EDTA and dithiothreitol, but only slightly affected by?NaN3?and xylene. The spore enzyme efficiently decolorized?anthraquinone and azo dyes?in 24 h.
机译：使用补充了0.4 mmol / L Cu2 +的M9培养基从森林土壤中分离出一种名为WD23的细菌菌株。来自该菌株的孢子表现出漆酶样活性，氧化丁香醛嗪，2，6-二甲氧基苯酚和α2，2′-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）。根据其形态和生理特性以及16S rDNA序列分析，该菌株被鉴定为枯草芽孢杆菌。结合孢子的漆酶的最适pH和温度分别为约6.8和60℃。漆酶的温度半衰期在约80℃下为2.5小时，在约60℃下为68小时。在宽pH范围内也显示出高稳定性。在pH 6.8下，“漆酶”的pH半衰期超过6个月。测试了漆酶对有机溶剂和抑制剂的敏感性，发现漆酶的活性受到甲醇，EDTA和二硫苏糖醇的强烈抑制，而对NaN3和二甲苯的影响很小。孢子酶可在24小时内有效地使蒽醌和偶氮染料脱色。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号